PDF(Pubmed)
Abstract：
UNASSIGNED: Due to their capacity to release growth factors and cytokines, co-culture using mesenchymal stem cells has been considered a good alternative to promoting the maturation of the oocytes and the embryo\'s development quality in vitro in different mammalian species. In this regard, we investigated the effect of feline Wharton\'s jelly MSCs as feeders layer in oocyte maturation-consequently, the development of resulting embryos in co-culture.
UNASSIGNED: Oocytes with dark cytoplasm and a few layers of cumulus cells were collected and subjected to in vitro maturation and embryo culture using commercial media with and without MSCs addition. The oocytes\' nuclear maturation and the degree of cumulus expansion in different groups were assessed after 24 h; the development of the embryo was evaluated every 12 h until day eight.
UNASSIGNED: Although MSCs increased the proportion of cumulus cells oocytes exhibiting cumulus expansion, there were no significant differences in the percentage of matured oocytes (metaphase II) among the groups (p > 0.05). However, the embryo development differs significantly, with a higher cleavage, morula, and blastocyst percentage in oocytes matured with MSC co-culture conditions than in commercial media alone (p < 0.05). Also, we observed higher morula and blastocyst rates in the embryos co-cultured with MSCs during the in vitro culture (p > 0.05).
UNASSIGNED: Based on our results, the co-culture with MSCs during the oocyte maturation resulted in better embryo development, as well as the MSCs addition during embryo culture returned an increased number of morula and blastocysts. Further research is needed to fully understand and optimize the use of MSCs in oocyte maturation and embryo development.
UNASSIGNED: Oocytes with dark cytoplasm and a few layers of cumulus cells were collected and subjected to in vitro maturation and embryo culture using commercial media with and without MSCs addition. The oocytes\' nuclear maturation and the degree of cumulus expansion in different groups were assessed after 24 h; the development of the embryo was evaluated every 12 h until day eight.
UNASSIGNED: Although MSCs increased the proportion of cumulus cells oocytes exhibiting cumulus expansion, there were no significant differences in the percentage of matured oocytes (metaphase II) among the groups (p > 0.05). However, the embryo development differs significantly, with a higher cleavage, morula, and blastocyst percentage in oocytes matured with MSC co-culture conditions than in commercial media alone (p < 0.05). Also, we observed higher morula and blastocyst rates in the embryos co-cultured with MSCs during the in vitro culture (p > 0.05).
UNASSIGNED: Based on our results, the co-culture with MSCs during the oocyte maturation resulted in better embryo development, as well as the MSCs addition during embryo culture returned an increased number of morula and blastocysts. Further research is needed to fully understand and optimize the use of MSCs in oocyte maturation and embryo development.
摘要：
■由于它们释放生长因子和细胞因子的能力，使用间充质干细胞共培养被认为是促进卵母细胞成熟和胚胎发育质量的良好替代方法。在这方面,我们研究了猫科动物Wharton的果冻MSCs作为饲养层在卵母细胞成熟中的作用，共培养中所得胚胎的发育。
■收集具有深色细胞质和几层卵丘细胞的卵母细胞,并使用添加和不添加MSC的商业培养基进行体外成熟和胚胎培养。24小时后评估不同组的卵母细胞核成熟和卵丘扩张程度；每12小时评估胚胎的发育，直到第8天。
■尽管MSCs增加了卵丘细胞的比例，各组间成熟卵母细胞（中期II）的百分比没有显着差异（p>0.05）。然而,胚胎发育差异显著,有更高的乳沟，Morula,与单独的商业培养基相比,在MSC共培养条件下成熟的卵母细胞中的囊胚百分比(p<0.05)。此外，在体外培养过程中，我们观察到与MSCs共培养的胚胎中桑形态和囊胚率较高（p>0.05）。
■根据我们的结果，在卵母细胞成熟过程中与MSCs共培养导致更好的胚胎发育，以及在胚胎培养过程中添加的MSCs返回了数量增加的桑形态和囊胚。需要进一步的研究来充分了解和优化MSCs在卵母细胞成熟和胚胎发育中的应用。
■收集具有深色细胞质和几层卵丘细胞的卵母细胞,并使用添加和不添加MSC的商业培养基进行体外成熟和胚胎培养。24小时后评估不同组的卵母细胞核成熟和卵丘扩张程度；每12小时评估胚胎的发育，直到第8天。
■尽管MSCs增加了卵丘细胞的比例，各组间成熟卵母细胞（中期II）的百分比没有显着差异（p>0.05）。然而,胚胎发育差异显著,有更高的乳沟，Morula,与单独的商业培养基相比,在MSC共培养条件下成熟的卵母细胞中的囊胚百分比(p<0.05)。此外，在体外培养过程中，我们观察到与MSCs共培养的胚胎中桑形态和囊胚率较高（p>0.05）。
■根据我们的结果，在卵母细胞成熟过程中与MSCs共培养导致更好的胚胎发育，以及在胚胎培养过程中添加的MSCs返回了数量增加的桑形态和囊胚。需要进一步的研究来充分了解和优化MSCs在卵母细胞成熟和胚胎发育中的应用。
