PDF(Pubmed)
Abstract：
UNASSIGNED: The taxonomic group of non-tuberculous mycobacteria (NTM) encompasses more than 190 species and subspecies, some of which can cause pulmonary and extrapulmonary diseases across various age groups in humans. However, different subspecies exhibit differential drug sensitivities, and traditional detection techniques struggle to accurately classify NTM. Therefore, clinicians need more effective detection methods to identify NTM subtypes, thus providing personalized medication for patients.
UNASSIGNED: We present the case of a 47-year-old female patient diagnosed with an intraabdominal infection caused by Mycobacterium syngnathidarum. Despite computed tomography of the chest suggesting potential tuberculosis, tuberculosis infection was ruled out due to negative TB-DNA results for ascites fluid and sputum and limited improvement of lung lesions after treatment. Additionally, acid-fast staining and Lowenstein-Jensen culture results revealed the presence of mycobacterium in ascites fluid. Subsequent whole-genome sequencing (WGS) confirmed the DNA sequences of Mycobacterium syngnathidarum in colonies isolated from the ascites fluid, which was further corroborated by polymerase chain reaction and Sanger sequencing. Ultimately, the patient achieved a complete recovery following the treatment regimen targeting Mycobacterium syngnathidarum, which involved clarithromycin, ethambutol hydrochloride, pyrazinamide, rifampicin, and isoniazid.
UNASSIGNED: This is the first reported case of Mycobacterium syngnathidarum infection in humans. Mycobacterium syngnathidarum was detected by WGS in this case, suggesting that WGS may serve as a high-resolution assay for the diagnosis of different subtypes of mycobacterium infection.
UNASSIGNED: We present the case of a 47-year-old female patient diagnosed with an intraabdominal infection caused by Mycobacterium syngnathidarum. Despite computed tomography of the chest suggesting potential tuberculosis, tuberculosis infection was ruled out due to negative TB-DNA results for ascites fluid and sputum and limited improvement of lung lesions after treatment. Additionally, acid-fast staining and Lowenstein-Jensen culture results revealed the presence of mycobacterium in ascites fluid. Subsequent whole-genome sequencing (WGS) confirmed the DNA sequences of Mycobacterium syngnathidarum in colonies isolated from the ascites fluid, which was further corroborated by polymerase chain reaction and Sanger sequencing. Ultimately, the patient achieved a complete recovery following the treatment regimen targeting Mycobacterium syngnathidarum, which involved clarithromycin, ethambutol hydrochloride, pyrazinamide, rifampicin, and isoniazid.
UNASSIGNED: This is the first reported case of Mycobacterium syngnathidarum infection in humans. Mycobacterium syngnathidarum was detected by WGS in this case, suggesting that WGS may serve as a high-resolution assay for the diagnosis of different subtypes of mycobacterium infection.
摘要：
■非结核分枝杆菌（NTM）的分类群包括190多个物种和亚种，其中一些会导致不同年龄段的肺部和肺外疾病。然而,不同的亚种表现出不同的药物敏感性，传统的检测技术难以对NTM进行准确分类。因此,临床医生需要更有效的检测方法来识别NTM亚型，从而为患者提供个性化用药。
■我们介绍了一名47岁的女性患者的病例，该患者被诊断为由合颌分枝杆菌引起的腹腔感染。尽管胸部计算机断层扫描显示潜在的结核病，由于腹水和痰液的TB-DNA结果阴性以及治疗后肺部病变的改善有限,结核感染被排除.此外,抗酸染色和Lowenstein-Jensen培养结果显示腹水中存在分枝杆菌。随后的全基因组测序（WGS）证实了从腹水中分离出的菌落中的联合分枝杆菌的DNA序列，聚合酶链反应和桑格测序进一步证实了这一点。最终,患者在采用联合分枝杆菌治疗方案后完全恢复，其中涉及克拉霉素，盐酸乙胺丁醇,吡嗪酰胺,利福平,还有异烟肼.
■这是人类中首例报道的合下分枝杆菌感染病例。在这种情况下，通过WGS检测到了合颌分枝杆菌，提示WGS可作为诊断不同亚型分枝杆菌感染的高分辨率检测方法。
■我们介绍了一名47岁的女性患者的病例，该患者被诊断为由合颌分枝杆菌引起的腹腔感染。尽管胸部计算机断层扫描显示潜在的结核病，由于腹水和痰液的TB-DNA结果阴性以及治疗后肺部病变的改善有限,结核感染被排除.此外,抗酸染色和Lowenstein-Jensen培养结果显示腹水中存在分枝杆菌。随后的全基因组测序（WGS）证实了从腹水中分离出的菌落中的联合分枝杆菌的DNA序列，聚合酶链反应和桑格测序进一步证实了这一点。最终,患者在采用联合分枝杆菌治疗方案后完全恢复，其中涉及克拉霉素，盐酸乙胺丁醇,吡嗪酰胺,利福平,还有异烟肼.
■这是人类中首例报道的合下分枝杆菌感染病例。在这种情况下，通过WGS检测到了合颌分枝杆菌，提示WGS可作为诊断不同亚型分枝杆菌感染的高分辨率检测方法。
