PDF(Pubmed)
Abstract：
UNASSIGNED: Ureteral injuries require surgical intervention as they lead to loss of renal function. The current reconstructive techniques for long ureteral defects are problematic. Consequently, this study aimed to reconstruct the ureter in a rat model using subcutaneously prepared autologous collagen tubes (Biotubes).
UNASSIGNED: The lower ureter of LEW/SsNSlc rats was ligated to dilate the ureter to make anastomosis easier, and reconstruction was performed six days later by anastomosing the dilated ureter and bladder with a Biotube that was prepared subcutaneously in syngeneic rats. Some rats underwent left nephrectomy and ureter reconstruction simultaneously as negative controls to evaluate the effects of urine flow on patency. The other rats were divided into three groups as follows: a group in which the ureter was reconstructed with the Biotube alone, a group in which cardiomyocyte sheets made from the neonatal hearts of syngeneic rats were wrapped around the Biotube, and a group in which an adipose-derived stem cell sheets made from the inguinal fat of adult syngeneic rats were wrapped. Contrast-enhanced computed tomography and pathological evaluations were performed two weeks after reconstruction.
UNASSIGNED: In the Biotube alone group, all tubes were occluded and hydronephrosis developed, whereas the urothelium regenerated beyond the anastomosis when the left kidney was not removed, suggesting that urothelial epithelial spread occurred with urinary flow. The patency of the ureteral lumen was obtained in some rats in the cardiomyocyte sheet covered group, whereas stricture or obstruction of the reconstructed ureter was observed in all rats in the other groups. Pathological evaluation revealed a layered urothelial structure in the cardiomyocyte sheet covered group, although only a small amount of cardiomyocyte sheets remained.
UNASSIGNED: Urinary flow may support the epithelial spread of the urothelium into the reconstructed ureter. Neonatal rat cardiomyocyte sheets supported the patency of the regenerated ureter with a layered urothelium.
UNASSIGNED: The lower ureter of LEW/SsNSlc rats was ligated to dilate the ureter to make anastomosis easier, and reconstruction was performed six days later by anastomosing the dilated ureter and bladder with a Biotube that was prepared subcutaneously in syngeneic rats. Some rats underwent left nephrectomy and ureter reconstruction simultaneously as negative controls to evaluate the effects of urine flow on patency. The other rats were divided into three groups as follows: a group in which the ureter was reconstructed with the Biotube alone, a group in which cardiomyocyte sheets made from the neonatal hearts of syngeneic rats were wrapped around the Biotube, and a group in which an adipose-derived stem cell sheets made from the inguinal fat of adult syngeneic rats were wrapped. Contrast-enhanced computed tomography and pathological evaluations were performed two weeks after reconstruction.
UNASSIGNED: In the Biotube alone group, all tubes were occluded and hydronephrosis developed, whereas the urothelium regenerated beyond the anastomosis when the left kidney was not removed, suggesting that urothelial epithelial spread occurred with urinary flow. The patency of the ureteral lumen was obtained in some rats in the cardiomyocyte sheet covered group, whereas stricture or obstruction of the reconstructed ureter was observed in all rats in the other groups. Pathological evaluation revealed a layered urothelial structure in the cardiomyocyte sheet covered group, although only a small amount of cardiomyocyte sheets remained.
UNASSIGNED: Urinary flow may support the epithelial spread of the urothelium into the reconstructed ureter. Neonatal rat cardiomyocyte sheets supported the patency of the regenerated ureter with a layered urothelium.
摘要：
■输尿管损伤需要手术干预，因为它们导致肾功能丧失。当前用于长输尿管缺损的重建技术存在问题。因此,这项研究旨在使用皮下制备的自体胶原管（Biotube）重建大鼠模型中的输尿管。
■将LEW/SsNSlc大鼠的下输尿管结扎以扩张输尿管，使吻合更容易，六天后，通过用同种异体大鼠皮下制备的Biotube吻合扩张的输尿管和膀胱进行重建。部分大鼠同时行左肾切除和输尿管重建术作为阴性对照,以评价尿流对通畅性的影响。其他大鼠分为以下三组：一组仅使用Biotube重建输尿管，一组由同基因大鼠的新生心脏制成的心肌细胞被包裹在Biotube上，和一组由成年同基因大鼠腹股沟脂肪制成的脂肪干细胞片被包裹。重建后两周进行对比增强计算机断层扫描和病理评估。
■在单独的Biotube组中，所有的管道都闭塞，肾积水发展，而当左肾未切除时，尿路上皮在吻合口之外再生，提示尿路上皮与尿流一起扩散。心肌细胞片覆盖组部分大鼠输尿管管腔通畅,而在其他组的所有大鼠中均观察到重建输尿管的狭窄或阻塞。病理评估显示在心肌细胞片覆盖组中有分层的尿路上皮结构，尽管只剩下少量的心肌细胞片。
■尿流可能支持尿路上皮向重建输尿管的上皮扩散。新生大鼠心肌细胞片以层状尿路上皮支持再生输尿管的通畅。
■将LEW/SsNSlc大鼠的下输尿管结扎以扩张输尿管，使吻合更容易，六天后，通过用同种异体大鼠皮下制备的Biotube吻合扩张的输尿管和膀胱进行重建。部分大鼠同时行左肾切除和输尿管重建术作为阴性对照,以评价尿流对通畅性的影响。其他大鼠分为以下三组：一组仅使用Biotube重建输尿管，一组由同基因大鼠的新生心脏制成的心肌细胞被包裹在Biotube上，和一组由成年同基因大鼠腹股沟脂肪制成的脂肪干细胞片被包裹。重建后两周进行对比增强计算机断层扫描和病理评估。
■在单独的Biotube组中，所有的管道都闭塞，肾积水发展，而当左肾未切除时，尿路上皮在吻合口之外再生，提示尿路上皮与尿流一起扩散。心肌细胞片覆盖组部分大鼠输尿管管腔通畅,而在其他组的所有大鼠中均观察到重建输尿管的狭窄或阻塞。病理评估显示在心肌细胞片覆盖组中有分层的尿路上皮结构，尽管只剩下少量的心肌细胞片。
■尿流可能支持尿路上皮向重建输尿管的上皮扩散。新生大鼠心肌细胞片以层状尿路上皮支持再生输尿管的通畅。
