PDF(Pubmed)
Abstract：
UNASSIGNED: To examine the common types of hemoglobin variants and to evaluate the influence of common variants on the results of two kinds of glycosylated hemoglobin (HbA1c) tests.
UNASSIGNED: We conducted a retrospective study, analyzing the data of a patient population undergoing two HbA1c tests, high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), at West China Hospital, Sichuan University between March 2021 and February 2022. By screening the chromatograms, the hemoglobin variants were identified and their migration positions in the CE method were recorded. The effects of the variants with different migration positions on the findings of the two methods were compared. Variant samples with different migration positions were selected and Sanger sequencing was performed to determine mutations in HBA1, HBA2, and HBB genes in the variant samples.
UNASSIGNED: We examined the HbA1c of 207 786 patient samples, identifying variant peaks in the chromatograms of 372 patients. The detection rate of variants was 0.18%, with the variant identification rate of HPLC being 43.3% and that of CE, 100%. Through sequencing, 20 variants were detected. A total of 261 patient samples were tested for HbA1c with both HPLC and CE. HPLC reported all HbA1c results, while CE did not report HbA1c results for 28 samples, among which, 26 showed abnormal peaks that overlapped with HbA1c peaks, and 2 showed abnormal peaks that overlapped with HbA0 peaks. The commonly observed variant migration positions, as revealed by CE, were at the horizontal coordinates of 225±1, 200±3, 100±2, 124±1, 70±2, and 182±1. There was significant difference between HPLC method and CE method in the determination of HbA1c ( P<0.0083), and the difference between the two methods was the largest when there were variants in the 200±3 region. Linear regression showed that the correlation of HbA1c results between the two methods was different when different regional variants were present, and that the correlation between the two methods was strongest when 124±1 region was present ( r=0.998).
UNASSIGNED: There are diverse types of hemoglobin variants and most of them can affect the HbA1c findings of HPLC. Analyzing the chromatogram facilitates the identification of the variants.
UNASSIGNED: We conducted a retrospective study, analyzing the data of a patient population undergoing two HbA1c tests, high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), at West China Hospital, Sichuan University between March 2021 and February 2022. By screening the chromatograms, the hemoglobin variants were identified and their migration positions in the CE method were recorded. The effects of the variants with different migration positions on the findings of the two methods were compared. Variant samples with different migration positions were selected and Sanger sequencing was performed to determine mutations in HBA1, HBA2, and HBB genes in the variant samples.
UNASSIGNED: We examined the HbA1c of 207 786 patient samples, identifying variant peaks in the chromatograms of 372 patients. The detection rate of variants was 0.18%, with the variant identification rate of HPLC being 43.3% and that of CE, 100%. Through sequencing, 20 variants were detected. A total of 261 patient samples were tested for HbA1c with both HPLC and CE. HPLC reported all HbA1c results, while CE did not report HbA1c results for 28 samples, among which, 26 showed abnormal peaks that overlapped with HbA1c peaks, and 2 showed abnormal peaks that overlapped with HbA0 peaks. The commonly observed variant migration positions, as revealed by CE, were at the horizontal coordinates of 225±1, 200±3, 100±2, 124±1, 70±2, and 182±1. There was significant difference between HPLC method and CE method in the determination of HbA1c ( P<0.0083), and the difference between the two methods was the largest when there were variants in the 200±3 region. Linear regression showed that the correlation of HbA1c results between the two methods was different when different regional variants were present, and that the correlation between the two methods was strongest when 124±1 region was present ( r=0.998).
UNASSIGNED: There are diverse types of hemoglobin variants and most of them can affect the HbA1c findings of HPLC. Analyzing the chromatogram facilitates the identification of the variants.
摘要：
■检查血红蛋白变体的常见类型，并评估常见变体对两种糖基化血红蛋白（HbA1c）测试结果的影响。
■我们进行了一项回顾性研究，分析接受两次HbA1c测试的患者人群的数据，高效液相色谱（HPLC）和毛细管电泳（CE），在华西医院，四川大学，2021年3月至2022年2月。通过筛选色谱图，确定血红蛋白变异体,并记录其在CE方法中的迁移位置.比较了具有不同迁移位置的变体对两种方法的发现的影响。选择具有不同迁移位置的变体样品并进行Sanger测序以确定变体样品中HBA1、HBA2和HBB基因的突变。
■我们检查了207786例患者样本的HbA1c，在372名患者的色谱图中鉴定变异峰。变异体的检出率为0.18%,HPLC的变异识别率为43.3%，CE的变异识别率为43.3%，100%。通过测序，检测到20个变异。用HPLC和CE测试总共261个患者样品的HbA1c。HPLC报告了所有HbA1c结果，虽然CE没有报告28个样本的HbA1c结果，其中,26显示与HbA1c峰重叠的异常峰，和2显示与HbA0峰重叠的异常峰。通常观察到的变异迁移位置，据CE透露，位于225±1、200±3、100±2、124±1、70±2和182±1的水平坐标处。HPLC法和CE法测定HbA1c有显著性差异(P<0.0083),当200±3区域存在变异时，两种方法的差异最大。线性回归表明，当存在不同的区域变异时，两种方法之间的HbA1c结果的相关性不同。当存在124±1个区域时，两种方法之间的相关性最强（r=0.998）。
■血红蛋白变体的类型多种多样，其中大多数可以影响HPLC的HbA1c发现。分析色谱有助于变体的鉴定。
■我们进行了一项回顾性研究，分析接受两次HbA1c测试的患者人群的数据，高效液相色谱（HPLC）和毛细管电泳（CE），在华西医院，四川大学，2021年3月至2022年2月。通过筛选色谱图，确定血红蛋白变异体,并记录其在CE方法中的迁移位置.比较了具有不同迁移位置的变体对两种方法的发现的影响。选择具有不同迁移位置的变体样品并进行Sanger测序以确定变体样品中HBA1、HBA2和HBB基因的突变。
■我们检查了207786例患者样本的HbA1c，在372名患者的色谱图中鉴定变异峰。变异体的检出率为0.18%,HPLC的变异识别率为43.3%，CE的变异识别率为43.3%，100%。通过测序，检测到20个变异。用HPLC和CE测试总共261个患者样品的HbA1c。HPLC报告了所有HbA1c结果，虽然CE没有报告28个样本的HbA1c结果，其中,26显示与HbA1c峰重叠的异常峰，和2显示与HbA0峰重叠的异常峰。通常观察到的变异迁移位置，据CE透露，位于225±1、200±3、100±2、124±1、70±2和182±1的水平坐标处。HPLC法和CE法测定HbA1c有显著性差异(P<0.0083),当200±3区域存在变异时，两种方法的差异最大。线性回归表明，当存在不同的区域变异时，两种方法之间的HbA1c结果的相关性不同。当存在124±1个区域时，两种方法之间的相关性最强（r=0.998）。
■血红蛋白变体的类型多种多样，其中大多数可以影响HPLC的HbA1c发现。分析色谱有助于变体的鉴定。
