PDF(Pubmed)
Abstract：
The utilization of guided tissue regeneration membranes is a significant approach for enhancing bone tissue growth in areas with bone defects. Biodegradable magnesium alloys are increasingly being used as guided tissue regeneration membranes due to their outstanding osteogenic properties. However, the degradation rates of magnesium alloy bone implants documented in the literature tend to be rapid. Moreover, many studies focus only on the initial 3-month period post-implantation, limiting their applicability and impeding clinical adoption. Furthermore, scant attention has been given to the interplay between the degradation of magnesium alloy implants and the adjacent tissues. To address these gaps, this study employs a well-studied magnesium-aluminum (Mg-Al) alloy membrane with a slow degradation rate. This membrane is implanted into rat skull bone defects and monitored over an extended period of up to 48 weeks. Observations are conducted at various intervals (2, 4, 8, 12, 24, and 48 weeks) following the implantation. Assessment of degradation behavior and tissue regeneration response is carried out using histological sections, micro-CT scans, and scanning electron microscopy (SEM). The findings reveal that the magnesium alloy membranes demonstrate remarkable biocompatibility and osteogenic capability over the entire observation duration. Specifically, the Mg-Al alloy membranes sustain their structural integrity for 8 weeks. Notably, their osteogenic ability is further enhanced as a corrosion product layer forms during the later stages of implantation. Additionally, our in vitro experiments employing extracts from the magnesium alloy display a significant osteogenic effect, accompanied by a notable increase in the expression of osteogenic-related genes. Collectively, these results strongly indicate the substantial potential of Mg-Al alloy membranes in the context of guided tissue regeneration.
摘要：
利用引导组织再生膜是增强骨缺损区域骨组织生长的重要方法。生物可降解镁合金由于其突出的成骨性能而越来越多地用作引导组织再生膜。然而,文献中记载的镁合金骨植入物的降解速率趋于快速。此外，许多研究只关注植入后最初的3个月，限制其适用性并阻碍临床采用。此外，镁合金植入物的降解与邻近组织之间的相互作用很少受到关注。为了弥补这些差距,这项研究采用了经过充分研究的镁铝（Mg-Al）合金膜，降解速率较慢。将该膜植入大鼠颅骨骨缺损中,并在长达48周的长时间内进行监测。在植入后以各种间隔(2、4、8、12、24和48周)进行观察。使用组织学切片进行降解行为和组织再生反应的评估，显微CT扫描,和扫描电子显微镜（SEM）。结果表明，在整个观察期间，镁合金膜表现出显著的生物相容性和成骨能力。具体来说,Mg-Al合金膜维持其结构完整性8周。值得注意的是,由于在植入的后期形成腐蚀产物层，它们的成骨能力进一步增强。此外,我们采用镁合金提取物的体外实验显示出显着的成骨作用，伴随着成骨相关基因的表达显着增加。总的来说，这些结果强烈表明Mg-Al合金膜在引导组织再生方面的巨大潜力。
