Abstract：
Noble metal has always been used as a preferred base for SERS substrate. However, the preparation cost of such materials is trully high. Therefore, many researchers have begun to search for succedanea which cost were lower. In this work, CsPbBr3@ZIF-8 was synthesized by in-situ reduction method and combined with graphene nanosheets to construct a SERS substrate. The SERS performance of this substrate could be further enhanced by the synergistic effect of perovskite quantum dots and graphene. Base on this material, a sensitive SERS strategy composed of CsPbBr3@ZIF-8@G, antibody, and Bradford method was developed for the quantitative determination of cardiac troponin I (cTnI) in human serum. It\'s worth noting that the sensitivity and accuracy of this method could approach the level of other SERS methods using noble metals. The \"reverse\"-SERS method could improve the uniformity and stability of detection platform obviously. The detection range of this method was 0.01-100 ng/mL, and the estimated detection of limit (LOD) was 4.7 pg/mL. The recovery rate of this method range was between 93.1 % and 104.8 %, and RSD range was between 4.47 % and 7.06 %.
摘要：
贵金属一直被用作SERS衬底的优选基底。然而,这种材料的制备成本非常高。因此,许多研究人员已经开始寻找成本更低的sucedanea。在这项工作中,通过原位还原法合成了CsPbBr3@ZIF-8，并与石墨烯纳米片结合构建了SERS基底。钙钛矿量子点和石墨烯的协同作用可以进一步增强该基底的SERS性能。基于这种材料，由CsPbBr3@ZIF-8@G组成的敏感SERS策略，抗体，并建立了定量测定人血清中肌钙蛋白I（cTnI）的Bradford方法。值得注意的是，该方法的灵敏度和准确性可以接近其他使用贵金属的SERS方法的水平。“反向”-SERS方法可以明显提高检测平台的均匀性和稳定性。该方法的检测范围为0.01-100ng/mL，估计检出限（LOD）为4.7pg/mL。该方法的回收率在93.1%~104.8%之间,RSD范围为4.47%～7.06%。
