Abstract：
This study aimed to identify genomic regions, pathways, and putative candidate genes associated with resistance to gastrointestinal nematode in Santa Ines sheep. The phenotypic information comprised 5529 records from 1703 naturally infected animals. After genomic data quality control, 37,511 SNPs from 589 animals were available. The weighted single-step approach for genome-wide association study was performed to estimate the SNP effects and variances accounted by 10-SNP sliding windows. Confirming the polygenic nature of the studied traits, 20, 22, 21, and 19 genomic windows that explained more than 0.5% of the additive genetic variance were identified for fecal egg counts (FEC), Famacha© (FAM), packed cell volume (PCV), and total plasma protein (TPP), respectively. A total of 81, 122, 106, and 101 protein-coding genes were found in windows associated with FEC, FAM, PCV, and TPP, respectively. Several protein-coding genes related to the immune system and inflammatory response functions were identified within those genomic regions, such as ADCY9, ADRB2, BRAF, CADM1, CCL20, CD70, CREBBP, FNBP1, HTR4, IL16, IL22, IL26, MAPK8, NDFIP1, NLRC3, PAK5, PLCB1, PLCB4, ROCK1, TEK, TNFRSF12A, and VAV1. Functional enrichment analysis by DAVID tool also revealed many significant (P < 0.05) pathways and Gene Ontology terms that could be related to resistance to gastrointestinal nematode in Santa Ines sheep, such as chemokine signaling pathway (oas04062), cAMP signaling pathway (oas04024), cGMP-PKG signaling pathway (Oas04022), platelet activation (Oas04611), Rap1 signaling pathway (oas04015), and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen (GO:0016705). These results contribute to improving the knowledge of the genetic architecture of resistance to gastrointestinal nematode in Santa Ines sheep.
摘要：
这项研究旨在确定基因组区域，通路,以及与SantaInes绵羊对胃肠道线虫的抗性相关的假定候选基因。表型信息包括来自1703个自然感染动物的5529个记录。基因组数据质量控制后，可获得来自589只动物的37,511个SNP。进行用于全基因组关联研究的加权单步方法以估计SNP效应和由10-SNP滑动窗口所占的方差。确认所研究性状的多基因性质，20，22，21和19基因组窗口，解释了超过0.5％的加性遗传变异被确定为粪便卵数（FEC），Famacha©（FAM），细胞体积（PCV），和总血浆蛋白（TPP），分别。在与FEC相关的窗口中发现了总共81、122、106和101个蛋白质编码基因，FAM,PCV,TPP,分别。在这些基因组区域内鉴定了一些与免疫系统和炎症反应功能相关的蛋白质编码基因。如ADCY9，ADRB2，BRAF，CADM1,CCL20,CD70,CREBBP,FNBP1,HTR4,IL16,IL22,IL26,MAPK8,NDFIP1,NLRC3,PAK5,PLCB1,PLCB4,ROCK1,TEK,TNFRSF12A,和VAV1。通过DAVID工具进行的功能富集分析还揭示了许多显着（P<0.05）途径和基因本体论术语，这些术语可能与SantaInes绵羊对胃肠道线虫的抵抗力有关，如趋化因子信号通路(oas04062)，cAMP信号通路（oas04024），cGMP-PKG信号通路(Oas04022),血小板活化（Oas04611），Rap1信号通路(oas04015),和氧化还原酶活性，对配对的捐赠者采取行动，分子氧的掺入或还原（GO：0016705）。这些结果有助于提高对SantaInes绵羊对胃肠道线虫的抗性的遗传结构的认识。
