PDF(Pubmed)
Abstract：
Bursicon, a neuropeptide hormone comprising two subunits-bursicon (burs) and partner of burs (pburs), belongs to the cystine-knot protein family. Bursicon heterodimers and homodimers bind to the lucine-rich G-protein coupled receptor (LGR) encoded by rickets to regulate multiple physiological processes in arthropods. Notably, these processes encompass the regulation of female reproduction, a recent revelation in Tribolium castaneum. In this study we investigated the role of burs/pburs/rickets in mediating female vitellogenesis and reproduction in a hemipteran insect, the whitefly, Bemisia tabaci. Our investigation unveiled a synchronized expression of burs, pburs and rickets, with their transcripts persisting detectable in the days following eclosion. RNAi-mediated knockdown of burs, pburs or rickets significantly suppressed the transcript levels of vitellogenin (Vg) and Vg receptor in the female whiteflies. These effects also impaired ovarian maturation and female fecundity, as evidenced by a reduction in the number of eggs laid per female, a decrease in egg size and a decline in egg hatching rate. Furthermore, knockdown of burs, pburs or rickets led to diminished juvenile hormone (JH) titers and reduced transcript level of Kruppel homolog-1. However, this impact did not extend to genes in the insulin pathway or target of rapamycin pathway, deviating from the results observed in T. castaneum. Taken together, we conclude that burs/pburs/rickets regulates the vitellogenesis and reproduction in the whiteflies by coordinating with the JH signaling pathway.
摘要：
Bursicon,一种包含两个亚基的神经肽激素-Bursicon（Burs）和Burs的伴侣（Pburs），属于胱氨酸结蛋白家族。Bursicon异二聚体和同二聚体结合由of病编码的富含葡聚糖的G蛋白偶联受体（LGR），以调节节肢动物的多种生理过程。值得注意的是,这些过程包括调节雌性繁殖，Triboliumcastaneum最近的启示。在这项研究中，我们调查了在半翅目昆虫中，burs/pburs/病在介导雌性卵黄发生和繁殖中的作用。粉虱,烟粉虱.我们的调查揭示了Burs的同步表达，Pburs和病,它们的转录本在羽化后的几天内持续可检测到。RNAi介导的burs敲除，pburs或r病显着抑制了雌性粉虱中卵黄蛋白原（Vg）和Vg受体的转录水平。这些影响也损害了卵巢成熟和女性的繁殖力，每个雌性产卵数量的减少证明了这一点，卵大小的减少和卵孵化率的下降。此外，击倒Burs,pburs或病导致幼年激素（JH）滴度降低和Kruppel同源物1的转录水平降低。然而,这种影响并没有延伸到胰岛素途径或雷帕霉素途径的靶基因，偏离了在蓖麻中观察到的结果。一起来看,我们得出的结论是，Burs/pburs/r病通过与JH信号通路协调来调节粉虱的卵黄发生和繁殖。
