METHODS: Sixty patients were equally divided into three groups, Group I: Periodontally healthy, Group II: Non-smokers with Stage III periodontitis, and Group III: Smokers with Stage III periodontitis. The patients underwent NSPT with clinical and biochemical parameters examined at baseline and 3 months post therapy. GCF was collected for levels of SEMA-4D, PAD-2, and MMP-8 through enzyme-linked immunosorbent assay (ELISA).
RESULTS: Greater values of PPD (8.06 ± 0.19 mm), CAL (8.94 ± 0.19 mm), PI (2.58 ± 0.19) while lower PBI (1.39 ± 0.19%) and GI (1.72 ± 0.19) scores were seen in Group III as compared to Group II, which reduced significantly from baseline to 3 months in both the groups after NSPT. Minimum values of SEMA-4D, PAD-2, and MMP-8 levels in GCF were seen for Group I, which increased incrementally to Group II and III. Also, among Group II and III the SEMA-4D, PAD-2, and MMP-8 levels in GCF reduced from baseline to 3 months indicating a favorable response within the tissues.
CONCLUSIONS: Greater levels in GCF of Levels of SEMA-4D, PAD-2, and MMP-8 in Group II and III, which reduced significantly post NSPT, implied that these biomarkers play a pivotal role in the inflammatory process and can be utilized for early diagnosis.
方法:60例患者均分为3组,第一组:牙周健康,第二组:不吸烟者患有III期牙周炎,第三组:吸烟者患有III期牙周炎。患者在基线和治疗后3个月接受临床和生化参数检查的NSPT。收集GCF的SEMA-4D水平,PAD-2和MMP-8通过酶联免疫吸附测定(ELISA)。
结果:PPD值较大(8.06±0.19mm),CAL(8.94±0.19mm),与II组相比,III组的PI(2.58±0.19),而PBI(1.39±0.19%)和GI(1.72±0.19)得分较低,NSPT后,两组均从基线显着降低至3个月。SEMA-4D的最小值,GCF中的PAD-2和MMP-8水平见I组,逐步增加到第二组和第三组。此外,在第二组和第三组SEMA-4D中,GCF中的PAD-2和MMP-8水平从基线降低至3个月,表明组织内的良好反应。
结论:GCF中SEMA-4D水平较高,第二组和第三组的PAD-2和MMP-8,NSPT后显著减少,提示这些生物标志物在炎症过程中起关键作用,可用于早期诊断.