Abstract：
BACKGROUND: This study was designed to assess the influence of non-surgical periodontal therapy (NSPT) on gingival crevicular fluid (GCF) levels of semaphorin-4D (SEMA-4D), peptidylarginine deiminase-2 (PAD-2), and matrix metalloproteinase-8 (MMP-8) levels in periodontally healthy, Stage III periodontitis non-smoker and smoker patients.
METHODS: Sixty patients were equally divided into three groups, Group I: Periodontally healthy, Group II: Non-smokers with Stage III periodontitis, and Group III: Smokers with Stage III periodontitis. The patients underwent NSPT with clinical and biochemical parameters examined at baseline and 3 months post therapy. GCF was collected for levels of SEMA-4D, PAD-2, and MMP-8 through enzyme-linked immunosorbent assay (ELISA).
RESULTS: Greater values of PPD (8.06 ± 0.19 mm), CAL (8.94 ± 0.19 mm), PI (2.58 ± 0.19) while lower PBI (1.39 ± 0.19%) and GI (1.72 ± 0.19) scores were seen in Group III as compared to Group II, which reduced significantly from baseline to 3 months in both the groups after NSPT. Minimum values of SEMA-4D, PAD-2, and MMP-8 levels in GCF were seen for Group I, which increased incrementally to Group II and III. Also, among Group II and III the SEMA-4D, PAD-2, and MMP-8 levels in GCF reduced from baseline to 3 months indicating a favorable response within the tissues.
CONCLUSIONS: Greater levels in GCF of Levels of SEMA-4D, PAD-2, and MMP-8 in Group II and III, which reduced significantly post NSPT, implied that these biomarkers play a pivotal role in the inflammatory process and can be utilized for early diagnosis.
METHODS: Sixty patients were equally divided into three groups, Group I: Periodontally healthy, Group II: Non-smokers with Stage III periodontitis, and Group III: Smokers with Stage III periodontitis. The patients underwent NSPT with clinical and biochemical parameters examined at baseline and 3 months post therapy. GCF was collected for levels of SEMA-4D, PAD-2, and MMP-8 through enzyme-linked immunosorbent assay (ELISA).
RESULTS: Greater values of PPD (8.06 ± 0.19 mm), CAL (8.94 ± 0.19 mm), PI (2.58 ± 0.19) while lower PBI (1.39 ± 0.19%) and GI (1.72 ± 0.19) scores were seen in Group III as compared to Group II, which reduced significantly from baseline to 3 months in both the groups after NSPT. Minimum values of SEMA-4D, PAD-2, and MMP-8 levels in GCF were seen for Group I, which increased incrementally to Group II and III. Also, among Group II and III the SEMA-4D, PAD-2, and MMP-8 levels in GCF reduced from baseline to 3 months indicating a favorable response within the tissues.
CONCLUSIONS: Greater levels in GCF of Levels of SEMA-4D, PAD-2, and MMP-8 in Group II and III, which reduced significantly post NSPT, implied that these biomarkers play a pivotal role in the inflammatory process and can be utilized for early diagnosis.
摘要：
背景：本研究旨在评估非手术牙周治疗（NSPT）对信号素-4D（SEMA-4D）的龈沟液（GCF）水平的影响，肽基精氨酸脱亚胺酶-2（PAD-2），和基质金属蛋白酶-8（MMP-8）在牙周病健康，III期牙周炎非吸烟者和吸烟者。
方法：60例患者均分为3组，第一组：牙周健康，第二组：不吸烟者患有III期牙周炎，第三组：吸烟者患有III期牙周炎。患者在基线和治疗后3个月接受临床和生化参数检查的NSPT。收集GCF的SEMA-4D水平，PAD-2和MMP-8通过酶联免疫吸附测定(ELISA)。
结果：PPD值较大（8.06±0.19mm），CAL(8.94±0.19mm),与II组相比，III组的PI（2.58±0.19），而PBI（1.39±0.19％）和GI（1.72±0.19）得分较低，NSPT后，两组均从基线显着降低至3个月。SEMA-4D的最小值，GCF中的PAD-2和MMP-8水平见I组，逐步增加到第二组和第三组。此外，在第二组和第三组SEMA-4D中，GCF中的PAD-2和MMP-8水平从基线降低至3个月,表明组织内的良好反应。
结论：GCF中SEMA-4D水平较高，第二组和第三组的PAD-2和MMP-8，NSPT后显著减少，提示这些生物标志物在炎症过程中起关键作用,可用于早期诊断.
方法：60例患者均分为3组，第一组：牙周健康，第二组：不吸烟者患有III期牙周炎，第三组：吸烟者患有III期牙周炎。患者在基线和治疗后3个月接受临床和生化参数检查的NSPT。收集GCF的SEMA-4D水平，PAD-2和MMP-8通过酶联免疫吸附测定(ELISA)。
结果：PPD值较大（8.06±0.19mm），CAL(8.94±0.19mm),与II组相比，III组的PI（2.58±0.19），而PBI（1.39±0.19％）和GI（1.72±0.19）得分较低，NSPT后，两组均从基线显着降低至3个月。SEMA-4D的最小值，GCF中的PAD-2和MMP-8水平见I组，逐步增加到第二组和第三组。此外，在第二组和第三组SEMA-4D中，GCF中的PAD-2和MMP-8水平从基线降低至3个月,表明组织内的良好反应。
结论：GCF中SEMA-4D水平较高，第二组和第三组的PAD-2和MMP-8，NSPT后显著减少，提示这些生物标志物在炎症过程中起关键作用,可用于早期诊断.
