Abstract：
BACKGROUND: Upstream stimulatory factors (USFs) are members of the basic helix-loop-helix leucine zipper transcription factor family, including USF1, USF2, and USF3. The first two members have been well studied compared to the third member, USF3, which has received scarce attention in cancer research to date. Despite a recently reported association of its alteration with thyroid carcinoma, its expression has not been previously analyzed.
METHODS: We comprehensively analyzed differential levels of USFs expression, genomic alteration, DNA methylation, and their prognostic value across different cancer types and the possible correlation with tumor-infiltrating immune cells and drug response by using different bioinformatics tools.
RESULTS: Our findings established that USFs play an important role in cancers related to the urinary system and justify the necessity for further investigation. We implemented and offer a useful ShinyApp to facilitate researchers\' efforts to inquire about any other gene of interest and to perform the analysis of drug response in a user-friendly fashion at http://zzdlab.com:3838/Drugdiscovery/.
METHODS: We comprehensively analyzed differential levels of USFs expression, genomic alteration, DNA methylation, and their prognostic value across different cancer types and the possible correlation with tumor-infiltrating immune cells and drug response by using different bioinformatics tools.
RESULTS: Our findings established that USFs play an important role in cancers related to the urinary system and justify the necessity for further investigation. We implemented and offer a useful ShinyApp to facilitate researchers\' efforts to inquire about any other gene of interest and to perform the analysis of drug response in a user-friendly fashion at http://zzdlab.com:3838/Drugdiscovery/.
摘要：
背景：上游刺激因子（USF）是基本螺旋-环-螺旋亮氨酸拉链转录因子家族的成员，包括USF1、USF2和USF3。与第三位成员相比，前两位成员得到了很好的研究，USF3，迄今为止在癌症研究中很少受到关注。尽管最近有报道称其改变与甲状腺癌有关，它的表达以前没有被分析过。
方法：我们综合分析了USF表达的差异水平，基因组改变，DNA甲基化，通过使用不同的生物信息学工具，以及它们在不同癌症类型中的预后价值，以及与肿瘤浸润免疫细胞和药物反应的可能相关性。
结果：我们的发现证实了USF在与泌尿系统相关的癌症中起着重要作用，并证明了进一步研究的必要性。我们在http://zzdlab.com:3838/Drugdiscovery/上实施并提供有用的ShinyApp，以方便的方式帮助研究人员查询任何其他感兴趣的基因，并以用户友好的方式进行药物反应分析。
方法：我们综合分析了USF表达的差异水平，基因组改变，DNA甲基化，通过使用不同的生物信息学工具，以及它们在不同癌症类型中的预后价值，以及与肿瘤浸润免疫细胞和药物反应的可能相关性。
结果：我们的发现证实了USF在与泌尿系统相关的癌症中起着重要作用，并证明了进一步研究的必要性。我们在http://zzdlab.com:3838/Drugdiscovery/上实施并提供有用的ShinyApp，以方便的方式帮助研究人员查询任何其他感兴趣的基因，并以用户友好的方式进行药物反应分析。
