关键词: Epidermis Notochord Prdx Xenopus tropicalis

Mesh : Animals Xenopus Xenopus Proteins / genetics metabolism Real-Time Polymerase Chain Reaction Embryonic Development / genetics Gene Expression Regulation, Developmental Xenopus laevis / genetics

来  源:   DOI:10.1016/j.gep.2023.119345

Abstract:
Peroxidase genes (Prdx) encode a family of antioxidant proteins, which can protect cells from oxidative damage by reducing various cellular peroxides. This study investigated the spatiotemporal expression patterns of gene members in this family during the early development of Xenopus tropicalis. Real-time quantitative PCR showed that all members of this gene family have a distinct temporal expression pattern during the early development of X. tropicalis embryos. Additionally, whole mount in situ hybridization revealed that individual prdx genes display differential expression patterns, with overlapping expression in lymphatic vessels, pronephros, proximal tubule, and branchial arches. This study provides a basis for further study of the function of the prdx gene family.
摘要:
过氧化物酶基因(Prdx)编码抗氧化蛋白家族,它可以通过减少各种细胞过氧化物来保护细胞免受氧化损伤。本研究调查了该家族中基因成员在非洲爪螨早期发育过程中的时空表达模式。实时定量PCR表明,该基因家族的所有成员在X的早期发育过程中都具有不同的时间表达模式。热带肌胚胎。此外,整装原位杂交表明,单个prdx基因显示差异表达模式,在淋巴管中重叠表达,pronephros,近端小管,和branch弓。本研究为进一步研究prdx基因家族的功能提供了基础。
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