Abstract：
Genomic imprinting refers to the expression of parent-specific genes in diploid mammalian cells. MAGEL2 gene is a maternally imprinted gene that has been identified in mice and humans and is associated with the onset of puberty. The purpose of this study was to investigate its imprinting status and its relationship with the onset of puberty in Dolang sheep. The sequence of 3734 bp cDNA of MAGEL2 in Dolang sheep was obtained by cloning and sequencing, encoding 1173 amino acids. The results of the nucleotide and amino acid similarity analysis showed that it was highly conserved among different mammalian species. The MAGEL2 gene was expressed monoallelically in the tissues of adult and neonatal umbilical cords, and the expressed allele was paternally inherited. Real Time quantitative PCR (RT-qPCR) results showed that the MAGEL2 gene was highly expressed in the hypothalamus and pituitary gland, increased significantly from prepuberty to puberty, and decreased significantly after puberty. This study suggests that MAGEL2 is a paternally expressed and maternally imprinted gene in Dolang sheep, which may be involved in the initiation of puberty in Dolang sheep. This study provides a theoretical basis for further research on the mechanism of the imprinted gene MAGEL2 regulating the onset of puberty in sheep, and provides a new idea for the future research on the mechanism of onset of puberty in sheep.
摘要：
基因组印记是指亲本特异性基因在二倍体哺乳动物细胞中的表达。MAGEL2基因是一种母系印迹基因，已在小鼠和人类中鉴定，并与青春期的开始有关。目的探讨其印迹状况及其与多朗羊青春期开始的关系。通过克隆和测序获得了Dolang绵羊MAGEL2的3734bpcDNA序列，编码1173个氨基酸。核苷酸和氨基酸相似性分析的结果表明，它在不同哺乳动物物种之间高度保守。MAGEL2基因在成人和新生儿脐带组织中单等位基因表达，表达的等位基因是父系遗传的。实时定量PCR(RT-qPCR)结果显示,MAGEL2基因在下丘脑和垂体高表达,从青春期到青春期显着增加，青春期后明显下降。这项研究表明，MAGEL2是Dolang绵羊的父系表达和母系印迹基因，这可能与Dolang绵羊青春期的开始有关。本研究为进一步研究印迹基因MAGEL2调控绵羊青春期开始的机制提供了理论依据,为今后绵羊青春期发病机制的研究提供了新的思路。
