Abstract：
Bifidobacterium is a major probiotic of intestinal gut flora and exerts many physiological activities, and it is widely applied in the fields of food and medicine. As an important part of Bifidobacterium, glycoside hydrolase plays a role in its physiological activity. With the continuous development and improvement of genetic engineering technology, research on this type of enzyme will play a crucial role in promoting the further development of Bifidobacterium in the field of probiotics. In this review, the preparation methods, enzymatic properties, and functions of glycoside hydrolase extracted from Bifidobacterium are described and summarized. The common method for preparing glycoside hydrolase derived from Bifidobacterium is heterologous expression in Escherichia coli BL21. The optimal pH range for these glycoside hydrolase enzymes is between 4.5 and 7.5; the optimal temperature is between 30 and 50 °C, which is close to the optimal growth condition of Bifidobacterium. Based on substrate specificity, these glycoside hydrolase could hydrolyze synthetic substrates and natural oligosaccharides, including a series of pNP artificial substrates, disaccharide, and trisaccharides, while they have little ability to hydrolyze polysaccharide substrates. This review will be expected to provide a basis for the development of Bifidobacterium as a probiotic element.
摘要：
双歧杆菌是肠道菌群的主要益生菌,具有多种生理活性,广泛应用于食品和医药领域。作为双歧杆菌的重要组成部分,糖苷水解酶在其生理活性中起作用。随着基因工程技术的不断发展和完善,对这类酶的研究将对双歧杆菌在益生菌领域的进一步发展起到至关重要的推动作用。在这次审查中,制备方法，酶学性质，对从双歧杆菌中提取的糖苷水解酶的功能进行了描述和总结。制备源自双歧杆菌的糖苷水解酶的常用方法是在大肠杆菌BL21中异源表达。这些糖苷水解酶的最佳pH范围在4.5至7.5之间；最佳温度在30至50°C之间，接近双歧杆菌的最佳生长条件。基于底物特异性，这些糖苷水解酶可以水解合成底物和天然寡糖，包括一系列pNP人工基质，二糖，和三糖，而它们几乎没有能力水解多糖底物。本综述有望为双歧杆菌作为益生菌元素的开发提供依据。
