UNASSIGNED: We aimed to develop a clinical-grade PRS test suitable for BC risk-stratified screening with clinical recommendations and implementation in clinical practice.
UNASSIGNED: In the first phase of our study, we gathered previously published PRS models for predicting BC risk from the literature and validated them using the Estonian Biobank and UK Biobank data sets. We selected the best performing model based on prevalent data and independently validated it in both incident data sets. We then conducted absolute risk simulations, developed risk-based recommendations, and implemented the PRS test in clinical practice. In the second phase, we carried out a retrospective analysis of the PRS test\'s performance results in clinical practice.
UNASSIGNED: The best performing PRS included 2803 SNPs. The C-index of the Cox regression model associating BC status with PRS was 0.656 (SE = 0.05) with a hazard ratio of 1.66. The PRS can stratify individuals with more than a 3-fold risk increase. A total of 2637 BC PRS tests have been performed for women between the ages 30 and 83. Results in clinical use overlap well with expected PRS performance with 5.7% of women with more than 2-fold and 1.4% with more than 3-fold higher risk than the population average.
UNASSIGNED: The PRS test separates different BC risk levels and is feasible to implement in clinical practice.
■我们旨在开发一种适用于BC风险分层筛查的临床级PRS测试,并提供临床建议并在临床实践中实施。
■在我们研究的第一阶段,我们从文献中收集了以前发表的预测BC风险的PRS模型,并使用爱沙尼亚生物库和英国生物库数据集进行了验证.我们根据流行数据选择了性能最佳的模型,并在两个事件数据集中对其进行了独立验证。然后我们进行了绝对风险模拟,制定了基于风险的建议,并在临床实践中实施了PRS测试。在第二阶段,我们对PRS试验在临床实践中的表现结果进行了回顾性分析.
■表现最好的PRS包括2803个SNP。将BC状态与PRS相关联的Cox回归模型的C指数为0.656(SE=0.05),风险比为1.66。PRS可以对风险增加超过3倍的个体进行分层。对30至83岁的女性进行了总共2637次BCPRS测试。临床使用结果与预期的PRS表现很好地重叠,其中5.7%的女性比人口平均水平高2倍以上,1.4%的女性风险高3倍以上。
■PRS测试将不同的BC风险水平分开,并且在临床实践中实施是可行的。