PDF(Pubmed)
Abstract：
In India, limited studies are available on the epidemiological aspects of methicillin-resistant Staphylococcus aureus (MRSA) infections in both animal and human settings. Herein, we investigated the prevalence, antimicrobial resistance profile and molecular characteristics of MRSA isolates recovered from cattle using the One Health approach. Out of 66 mecA-positive staphylococci, species-specific multiplex PCR detected 24 % (n=16) of isolates as MRSA. Maximum antibiotic resistance was seen against cloxacillin (94 %, n=15) and least for enrofloxacin and cephalothin (each 13 %, n=2). Overall, 13 % (n=2) of MRSA isolates were multidrug-resistant. Molecular characterization by SCCmec typing identified 88 % (n=14) of MRSA isolates as type V. Twelve isolates (75 %) belonged to novel spa-type t17242, of which 67 % (n=8) belonged to agr type I. MLST analysis revealed ST 1687 (50 %, n=8) as the most predominant sequence type. Circulation of different MRSA clones among the cattle populace offers a risk of transmission to humans through direct contact, food chain or environmental contamination. Thus, continuous monitoring of MRSA strains is imperative for early diagnosis and for establishing effective treatment strategies to restrain the disease burden caused by MRSA infections.
摘要：
在印度,关于耐甲氧西林金黄色葡萄球菌(MRSA)感染在动物和人类环境中的流行病学方面的研究有限.在这里，我们调查了患病率，使用一种健康方法从牛中回收的MRSA分离株的抗菌素耐药性和分子特征。在66个mecA阳性葡萄球菌中,物种特异性多重PCR检测到24%(n=16)的MRSA分离株。对氯唑西林的最大抗生素耐药性(94%，n=15)，恩诺沙星和头孢菌素(各13%，n=2)。总的来说,13％（n=2）的MRSA分离株具有多重耐药性。通过SCCmec分型进行分子表征，将88%(n=14)的MRSA分离株鉴定为V型。12个分离株(75%)属于新型spa型t17242，其中67%(n=8)属于agr型I。MLST分析显示ST1687(50%，n=8)为最主要的序列类型。不同的MRSA克隆在牛人群中的循环存在通过直接接触传播给人类的风险，食物链或环境污染。因此，MRSA菌株的持续监测对于早期诊断和建立有效的治疗策略以抑制MRSA感染引起的疾病负担至关重要.
