Abstract：
The Macrobrachium rosenbergii industry is threatened by various Aeromonas, resulting in high mortality of adult prawns. However, there are few studies on the immune response of M. rosenbergii infected with Aeromonas dhakensis. In this study, we observed the hepatopancreas and gills histopathologically, performed a comparative transcriptome analysis of the hepatopancreas, and analyzed the candidate gene expression of immune-related genes in the hemolymph, hepatopancreas, and gills of M. rosenbergii that had been infected with A. dhakensis. Histopathology revealed the hepatopancreas was successively inflamed, followed by cellular vacuolation, lumen deformation, and finally tissue erosion; partial and severe inflammation of the gills occurred successively, and eventually the gill tissue atrophy and the gill filaments detached from the gill arch. Transcriptome analysis showed that a total of 77,742 unigenes and 8664 differentially expressed genes (DEGs), and the immune-related DEGs were mainly enriched in lysosome and phagosome pathways. In addition, 4 immune-related candidate genes (RhoA, CASP9, PKC, and DSCIGN) based on KEGG and PPI analysis were monitored at 6, 12, and 24h post injection (hpi) in hepatopancreas, hemolymph and gills. Their spatio-temporal expression results indicated that A. dhakensis have activated the immune system of M. rosenbergii. The present study may provide new information on the complex immune mechanism of M. rosenbergii.
摘要：
罗氏沼虾产业受到各种气单胞菌的威胁，导致成年对虾的高死亡率。然而,关于罗氏菌感染达克气单胞菌的免疫反应的研究很少。在这项研究中,我们在组织病理学上观察了肝胰腺和g，进行了肝胰腺的比较转录组分析，并分析了血淋巴中免疫相关基因的候选基因表达，肝胰腺,和感染了达克A.组织病理学显示肝胰腺相继发炎，随后是细胞空泡化，管腔变形,最后是组织侵蚀；相继发生了部分和严重的ill炎症，最终g组织萎缩，g细丝从g弓脱离。转录组分析表明，共有77,742个基因和8664个差异表达基因(DEGs)，免疫相关的DEGs主要富集在溶酶体和吞噬体途径中。此外,4个免疫相关候选基因(RhoA，CASP9,PKC,和DSCIGN）基于KEGG和PPI分析，在肝胰腺注射后6、12和24小时（hpi）进行监测，血淋巴和ill.它们的时空表达结果表明，达克曲霉菌已经激活了罗氏病菌的免疫系统。本研究可能为罗氏病菌的复杂免疫机制提供新的信息。
