PDF(Pubmed)
Abstract：
Soybean seed sugars are among the most abundant beneficial compounds for human and animal consumption in soybean seeds. Higher seed sugars such as sucrose are desirable as they contribute to taste and flavor in soy-based food. Therefore, the objectives of this study were to use the \'Forrest\' by \'Williams 82\' (F × W82) recombinant inbred line (RIL) soybean population (n = 309) to identify quantitative trait loci (QTLs) and candidate genes that control seed sugar (sucrose, stachyose, and raffinose) contents in two environments (North Carolina and Illinois) over two years (2018 and 2020). A total of 26 QTLs that control seed sugar contents were identified and mapped on 16 soybean chromosomes (chrs.). Interestingly, five QTL regions were identified in both locations, Illinois and North Carolina, in this study on chrs. 2, 5, 13, 17, and 20. Amongst 57 candidate genes identified in this study, 16 were located within 10 Megabase (MB) of the identified QTLs. Amongst them, a cluster of four genes involved in the sugars\' pathway was collocated within 6 MB of two QTLs that were detected in this study on chr. 17. Further functional validation of the identified genes could be beneficial in breeding programs to produce soybean lines with high beneficial sucrose and low raffinose family oligosaccharides.
摘要：
大豆种子糖是大豆种子中人类和动物消耗的最丰富的有益化合物之一。高级种子糖如蔗糖是理想的,因为它们有助于大豆基食品的味道和风味。因此,本研究的目的是使用Williams82（F×W82）重组自交系（RIL）大豆群体（n=309）的“Forrest”来鉴定数量性状基因座（QTLs）和候选基因控制种子糖（蔗糖，水苏糖,和棉子糖）在两个环境（北卡罗来纳州和伊利诺伊州）中的内容超过两年（2018年和2020年）。总共鉴定了26个控制种子糖含量的QTL，并将其定位在16个大豆染色体上（chrs。).有趣的是,在这两个地方都确定了五个QTL区域，伊利诺伊州和北卡罗来纳州，在这项关于chrs的研究中。2、5、13、17和20。在这项研究中确定的57个候选基因中，16个位于鉴定的QTL的10兆碱基(MB)内。在他们当中,参与糖途径的四个基因簇位于本研究中在chr上检测到的两个QTL的6MB内。17.鉴定的基因的进一步功能验证在育种程序中可能是有益的,以产生具有高有益蔗糖和低棉子糖家族寡糖的大豆品系。
