PDF(Pubmed)
Abstract：
The new trends in the consumption of table grapes and the growing interest in the environmental impact of this crop have pushed breeders toward the development of seedless cultivars endowed with resistance, through crossbreeding programs. To obtain seedless grapes, the use of embryo-rescue techniques is fundamental. In this research, a grape embryo-culture protocol was optimized and validated by using 39 cultivars and 41 cross-combinations carried out in the framework of a large private table grape program of the private network Italian Variety Club in the period 2017-2021 evaluating several factors, such as the improvement in embryo formation, germination and growth, and plantlet development. The embryo culture attitude of crosses between different combinations of seedless parents was assessed, and the rates of embryo development from the extracted ovules mostly ranged from 3.5 to 35.5% with 5 out of 43 genotypes outliers. Experiments conducted at different sampling times, in a range of 43-62 days after pollination (DAP), did not show significant differences between the samples analyzed, while the rate of embryos developed with the applied protocol proved its employability on multiple genotypes, although the grapevine genotype significantly influenced the technique efficiency.
摘要：
食用葡萄的新趋势以及对这种作物对环境的影响越来越感兴趣，这促使育种者朝着具有抗性的无核品种发展。通过杂交计划。为了获得无核葡萄，胚胎拯救技术的使用是至关重要的。在这项研究中,通过在2017-2021年期间在私人网络意大利品种俱乐部的大型私人鲜食葡萄计划的框架内进行的39个品种和41个交叉组合，对葡萄胚胎培养协议进行了优化和验证，评估了几个因素，例如胚胎形成的改善，发芽和生长，和植株发育。评估了无核亲本不同组合之间杂交的胚胎培养态度，提取的胚珠的胚胎发育率大多在3.5％至35.5％之间，43个基因型中有5个异常值。在不同采样时间进行的实验，在授粉后（DAP）的43-62天范围内，分析的样品之间没有显着差异，虽然应用方案开发的胚胎率证明了其在多种基因型上的适用性，尽管葡萄藤基因型显着影响技术效率。
