PDF(Pubmed)
Abstract：
Platycladus orientalis leaves are rich in flavonoids and polysaccharides, which offer high medicinal and nutritional benefits. This study aimed to investigate the impact of P. orientalis leaf extract (PLE) on the growth performance, fur quality, serum parameters, and intestinal microbiota of raccoon dogs. Sixty healthy male black raccoon dogs, aged 85 (±5) days, were randomly assigned to four groups and fed a basal diet supplemented with 0, 0.25, 0.50, and 1.00 g/kg PLE for 125 days (designated as groups P0, P1, P2, and P3, respectively). The results revealed that the raccoon dogs in group P1 exhibited increased average daily gain and underfur length while showing a decreased feed/gain ratio compared to group P0 (p < 0.05). However, the heart index in group P2 was significantly lower than in group P0 (p < 0.05), and the kidney index and serum alanine aminotransferase activities in group P3 were higher than in groups P2 and P0 (p < 0.05), suggesting potential adverse effects at higher PLE dosages. Notably, dietary PLE supplementation led to a reduction in serum glucose concentrations (p < 0.05), which may have implications for glucose regulation. Furthermore, the study explored the impact of dietary supplementation with 0.25 g/kg PLE on the raccoon dogs\' intestinal microbiota using high-throughput sequencing. The results showed significant alterations in the microbial community structure, with a notable decrease in the abundance of Prevotella copri in response to 0.25 g/kg PLE supplementation (p < 0.05). In conclusion, supplementing raccoon dogs\' diet with 0.25 g/kg PLE can lead to improved growth performance and a positive influence on the intestinal microbiota. However, caution should be exercised regarding higher dosages, as they may have adverse effects on certain parameters. As a result, PLE holds promise as a potential feed additive for fur animal production.
摘要：
侧柏叶富含类黄酮和多糖，提供了很高的医疗和营养益处。本研究旨在探讨侧柏叶提取物(PLE)对其生长性能的影响,毛皮质量,血清参数，和浣熊狗的肠道微生物群。60只健康的雄性黑浣熊狗,年龄85（±5）天，随机分为四组，并饲喂补充0、0.25、0.50和1.00g/kgPLE的基础日粮125天（分别指定为P0，P1，P2和P3组）。结果表明，P1组的浣熊犬表现出增加的平均日增重和下皮毛长度，同时表现出与P0组相比降低的饲料/增重比（p<0.05）。然而,P2组的心脏指数明显低于P0组（p<0.05），P3组的肾脏指数和血清谷丙转氨酶活性均高于P2组和P0组（p<0.05），提示在较高PLE剂量下的潜在不良反应。值得注意的是,膳食补充PLE导致血清葡萄糖浓度降低(p<0.05)，这可能对葡萄糖调节有影响。此外，本研究使用高通量测序方法探讨了饮食中添加0.25g/kgPLE对浣熊犬肠道微生物群的影响.结果表明，微生物群落结构发生了显著变化，对0.25g/kgPLE补充的反应，黄带普氏菌的丰度显着下降（p<0.05）。总之,用0.25g/kgPLE补充浣熊犬的饮食可以改善生长性能，并对肠道微生物群产生积极影响。然而,对于更高的剂量应该谨慎，因为它们可能对某些参数产生不利影响。因此,PLE有望成为毛皮动物生产的潜在饲料添加剂。
