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Abstract：
The present study investigated the insulin-like growth factors (IGFs) and their receptors and binding proteins among three pig breeds during weaning. Sixty Duroc (DR), Taoyuan black (TYB), and Xiangcun black (XCB) piglets (20 piglets per breed) were selected at 21 and 24 (3 days of post-weaning) days of age to analyze organ indices, plasma concentrations of IGF and IGF-binding proteins (IGFBPs) using ELISA kits, and gene expression of IGF-system-related components in different tissues. The plasma IGFBP-3 concentration in TYB piglets was higher (p > 0.05) than in the XCB and DR piglets at 21 days of age. At 21 days of age, compared with the DR piglets, the IGF-1 expression was lower (p < 0.05) in the kidney, but it was higher (p < 0.05) in the spleen of XCB and TYB piglets. At 24 days of age, the IGF-1 expression was higher (p < 0.05) in the kidney of TYB piglets than in the XCB and DR piglets, while IGFBP-3 in the stomach and IGFBP-4 in the liver of XCB and TYB piglets were lower (p < 0.05) compared with the DR piglets. Weaning down-regulated (p < 0.05) IGF-1 expression in the jejunum, spleen, and liver of piglets, while it up-regulated (p < 0.05) IGFBP-3 expression in the stomach, IGFBP-4 in the liver, IGFBP-5 in the ileum, and IGFBP-6 in the jejunum of DR piglets. Spearman\'s correlation analysis showed a negative correlation (p < 0.05) between plasma IGFBP-2 and IGFBP-5 concentration and the organ indices of piglets. Collectively, there were significant differences in the IGF system components among the three pig breeds. The IGF system components were altered during weaning, which might be involved in weaning stress to decrease the growth of piglets.
摘要：
本研究调查了断奶过程中三种猪品种的胰岛素样生长因子（IGFs）及其受体和结合蛋白。60杜洛克（DR），桃园黑(TYB),和香村黑（XCB）仔猪（每个品种20只仔猪）在21和24（断奶后3天）日龄进行器官指数分析，使用ELISA试剂盒，IGF和IGF结合蛋白（IGFBPs）的血浆浓度，以及IGF系统相关成分在不同组织中的基因表达。21日龄时TYB仔猪血浆IGFBP-3浓度高于XCB和DR仔猪(p>0.05)。在21天大的时候，与DR仔猪相比，IGF-1在肾脏中的表达较低（p<0.05），但在XCB和TYB仔猪的脾脏中含量较高（p<0.05）。在24天大的时候，TYB仔猪肾脏中IGF-1的表达高于XCB和DR仔猪(p<0.05),而XCB和TYB仔猪的胃中IGFBP-3和肝脏中IGFBP-4均低于DR仔猪(p<0.05)。断奶下调（p<0.05）空肠IGF-1表达，脾,脾和仔猪的肝脏，而它上调（p<0.05）胃中IGFBP-3的表达，肝脏中的IGFBP-4,回肠中的IGFBP-5，DR仔猪空肠中的IGFBP-6。Spearman相关分析显示,血浆IGFBP-2和IGFBP-5浓度与仔猪器官指数呈负相关(p<0.05)。总的来说，三个猪种之间的IGF系统成分存在显着差异。IGF系统成分在断奶过程中发生了改变，这可能与断奶应激降低仔猪生长有关。
