PDF(Pubmed)
Abstract：
Lipids are crucial components for the maintenance oof normal structure and function in the nervous system. Elucidating the diversity of lipids in spinal cords may contribute to our understanding of neurodevelopment. This study comprehensively analyzed the fatty acid (FA) compositions and lipidomes of the spinal cords of eight domesticated animal species: pig, cattle, yak, goat, horse, donkey, camel, and sika deer. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) were the primary FAs in the spinal cords of these domesticated animals, accounting for 72.54-94.23% of total FAs. Notably, oleic acid, stearic acid and palmitic acid emerged as the most abundant FA species. Moreover, untargeted lipidomics by UPLC-Q-Exactive Orbitrap-MS demonstrated that five lipid classes, including glycerophospholipids (GPs), sphingolipids (SPs), glycerolipids (GLs), FAs and saccharolipids (SLs), were identified in the investigated spinal cords, with phosphatidylcholine (PC) being the most abundant among all identified lipid classes. Furthermore, canonical correlation analysis showed that PC, PE, TAG, HexCer-NS and SM were significantly associated with genome sequence data. These informative data provide insight into the structure and function of mammalian nervous tissues and represent a novel contribution to lipidomics.
摘要：
脂质是维持神经系统正常结构和功能的关键成分。阐明脊髓中脂质的多样性可能有助于我们对神经发育的理解。本研究全面分析了8种家养动物的脊髓脂肪酸（FA）组成和脂质，牛,牦牛,山羊,马,驴,骆驼,还有梅花鹿.气相色谱-质谱（GC-MS）分析表明，饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）是这些家养动物脊髓中的主要FAs，占总FAs的72.54-94.23%。值得注意的是,油酸,硬脂酸和棕榈酸是最丰富的FA物种。此外，UPLC-Q-ExactiveOrbitrap-MS的非靶向脂质组学表明，五种脂质类别，包括甘油磷脂（GP），鞘脂（SP），甘油酯（GL），FAs和糖脂（SL），在被调查的脊髓中被发现，磷脂酰胆碱（PC）是所有确定的脂质类别中最丰富的。此外，典型相关分析表明，PC，PE,TAG,HexCer-NS和SM与基因组序列数据显著相关。这些信息数据提供了对哺乳动物神经组织的结构和功能的了解，并代表了对脂质组学的新贡献。
