PDF(Pubmed)
Abstract：
Innovative strategies have shown beneficial effects in healing wound management involving, however, a time-consuming and arduous process in clinical contexts. Micro-fragmented skin tissue acts as a slow-released natural scaffold and continuously delivers growth factors, and much other modulatory information, into the microenvironment surrounding damaged wounds by a paracrine function on the resident cells which supports the regenerative process. In this study, in vitro and in vivo investigations were conducted to ascertain improved effectiveness and velocity of the wound healing process with the application of fragmented dermo-epidermal units (FdeU), acquired via a novel medical device (Hy-Tissue® Micrograft Technology). MTT test; LDH test; ELISA for growth factor investigation (IL) IL-2, IL-6, IL-7 IL-8, IL-10; IGF-1; adiponectin; Fibroblast Growth Factor (FGF); Vascular Endothelial Growth Factor (VEGF); and Tumor Necrosis Factor (TNF) were assessed. Therefore, clinical evaluation in 11 patients affected by Chronic Wounds (CW) and treated with FdeU were investigated. Functional outcome was assessed pre-operatory, 2 months after treatment (T0), and 6 months after treatment (T1) using the Wound Bed Score (WBS) and Vancouver Scar Scale (VSS). In this current study, we demonstrate the potential of resident cells to proliferate from the clusters of FdeU seeded in a monolayer that efficiently propagate the chronic wound. Furthermore, in this study we report how the discharge of trophic/reparative proteins are able to mediate the in vitro paracrine function of proliferation, migration, and contraction rate in fibroblasts and keratinocytes. Our investigations recommend FdeU as a favorable tool in wound healing, displaying in vitro growth-promoting potential to enhance current therapeutic mechanisms.
摘要：
创新的策略已显示出有益的效果，在愈合伤口管理涉及，然而,在临床环境中，这是一个耗时而艰巨的过程。微破碎的皮肤组织作为一个缓慢释放的天然支架，不断输送生长因子，和许多其他调制信息，通过支持再生过程的常驻细胞上的旁分泌功能进入受损伤口周围的微环境。在这项研究中,进行了体外和体内研究，以确定使用碎裂的真皮表皮单位（FdeU）改善伤口愈合过程的有效性和速度，通过一种新型的医疗设备（Hy-Tissue®Micrograft技术）获得。MTT测试;LDH测试;用于生长因子研究的ELISA(IL)IL-2、IL-6、IL-7、IL-8、IL-10;IGF-1;脂联素;成纤维细胞生长因子(FGF);血管内皮生长因子(VEGF);和肿瘤坏死因子(TNF)被评估。因此,对11例慢性创伤(CW)患者的临床评估进行了研究,并接受了FdeU治疗.手术前评估功能结果，治疗后2个月（T0），和治疗后6个月（T1）使用伤口床评分（WBS）和温哥华疤痕量表（VSS）。在目前的研究中,我们证明了驻留细胞从单层接种的FdeU簇中增殖的潜力，该簇可以有效地传播慢性伤口。此外，在这项研究中，我们报告了营养/修复蛋白的放电如何能够介导体外旁分泌功能的增殖，迁移,和成纤维细胞和角质形成细胞的收缩率。我们的调查推荐FdeU作为伤口愈合的有利工具，显示体外促进生长的潜力，以增强当前的治疗机制。
