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Abstract：
Oxidoreductases are a wide class of enzymes that can catalyze biological oxidation and reduction reactions. Nowadays, oxidoreductases play a vital part in most bioenergetic metabolic pathways, which have important applications in biodegradation, bioremediation, environmental applications, as well as biosensors. However, free oxidoreductases are not stable and hard to be recycled. In addition, cofactors are needed in most oxidoreductases catalyze reactions, which are so expensive and unstable that it hinders their industrial applications. Enzyme immobilization is a feasible strategy that can overcome these problems. Recently, metal-organic frameworks (MOFs) have shown great potential as support materials for immobilizing enzymes due to their unique properties, such as high surface-area-to-volume ratio, chemical stability, functional designability, and tunable pore size. This review discussed the application of MOFs and their composites as immobilized carriers of oxidoreductase, as well as the application of MOFs as catalysts and immobilized carriers in redox reactions in the perspective of the function of MOFs materials. The paper also focuses on the potential of MOF carrier-based oxidoreductase immobilization for designing an enzyme cascade reaction system.
摘要：
氧化还原酶是可以催化生物氧化和还原反应的一类广泛的酶。如今,氧化还原酶在大多数生物能量代谢途径中起着至关重要的作用，它们在生物降解中有重要的应用，生物修复，环境应用,以及生物传感器。然而,游离的氧化还原酶不稳定，难以回收。此外,在大多数氧化还原酶催化反应中需要辅因子，它们是如此昂贵和不稳定，以至于阻碍了它们的工业应用。酶固定化是可以克服这些问题的可行策略。最近,金属有机框架（MOFs）由于其独特的性质，已显示出作为固定化酶的支持材料的巨大潜力，例如高表面积与体积比，化学稳定性,功能可设计性，和可调孔径。本文综述了MOFs及其复合材料作为氧化还原酶固定化载体的应用。以及MOFs作为催化剂和固定化载体在氧化还原反应中的应用，从MOFs材料的功能角度。本文还重点介绍了基于MOF载体的氧化还原酶固定化用于设计酶级联反应系统的潜力。
