PDF(Pubmed)
Abstract：
Plasmonic colorimetric sensors have emerged as powerful analytical tools in biochemistry due to their localized surface plasmon resonance extinction in the visible range. Here, we describe the feasibility of NAD(P)/NAD(P)H as redox agents in enzymatic plasmonic gold nanostar (AuNS) assays for galactose quantification using three model enzymes, GalDH, AR and GalOx, immobilized separately on polyvinylpyrrolidone-capped AuNS scaffolds. These highly specific, sensitive and selective bioassays induce the transformation of AuNS into quasi-spherical nanoparticles during the biorecognition of galactose in water and synthetic blood matrices. As a result, using our inexpensive and simple AuNS plasmon bioassays, the presence of galactose may be detected spectrophotometrically and by the naked eye.
摘要：
等离子体比色传感器由于其在可见范围内的局部表面等离子体共振消光而成为生物化学中强大的分析工具。这里,我们描述了NAD（P）/NAD（P）H作为氧化还原剂的可行性，在酶促等离子体金纳米星（AuNS）测定中使用三种模型酶进行半乳糖定量，GalDH,AR和GalOx,分别固定在聚乙烯吡咯烷酮封端的AuNS支架上。这些高度特定的，在水和合成血液基质中半乳糖的生物识别过程中，灵敏和选择性的生物测定会诱导AuNS转化为准球形纳米颗粒。因此,使用我们廉价而简单的AuNS等离子体生物测定法，半乳糖的存在可以通过分光光度法和肉眼检测。
