Abstract：
Periodontitis is one of the most prevalent chronic inflammatory diseases that may eventually lead to the loss of teeth. Macrophage polarization plays an important role in the development of periodontitis, and several naturally occurring food compounds have recently been reported to regulate macrophage polarization. In this study, we aimed to investigate the therapeutic potential of sulforaphene (SFE) in macrophage polarization and its impact on periodontitis. Through in vitro and in vivo experiments, our study demonstrated that SFE effectively inhibits M1 polarization while promoting M2 polarization, ultimately leading to the suppression of periodontitis. Transcriptome sequencing showed that SFE significantly upregulated the expression of dendritic cell immunoreceptor (DCIR, also known as CLEC4A2). We further validated the crucial role of DCIR in macrophage polarization through knockdown and overexpression experiments and demonstrated that SFE regulates macrophage polarization by upregulating DCIR expression. In summary, the results of this study suggest that SFE can regulate macrophage polarization and inhibit periodontitis. Moreover, this research identified DCIR (dendritic cell immunoreceptor) as a potential novel target for regulating macrophage polarization. These findings provide new insights into the treatment of periodontitis and other immune-related diseases.
摘要：
牙周炎是最常见的慢性炎症性疾病之一，最终可能导致牙齿脱落。巨噬细胞极化在牙周炎的发生发展中起重要作用,和几种天然存在的食物化合物最近被报道调节巨噬细胞极化。在这项研究中,我们旨在研究莱芬（SFE）在巨噬细胞极化中的治疗潜力及其对牙周炎的影响。通过体外和体内实验,我们的研究表明，SFE有效抑制M1极化，同时促进M2极化，最终导致牙周炎的抑制。转录组测序显示SFE显著上调树突状细胞免疫受体(DCIR,也称为CLEC4A2）。我们通过敲低和过表达实验进一步验证了DCIR在巨噬细胞极化中的关键作用，并证明SFE通过上调DCIR表达来调节巨噬细胞极化。总之,本研究结果提示SFE能调节巨噬细胞极化，抑制牙周炎。此外，这项研究确定DCIR（树突状细胞免疫受体）是调节巨噬细胞极化的潜在新靶标。这些发现为牙周炎和其他免疫相关疾病的治疗提供了新的见解。
