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Abstract：
Optimization and validation for simultaneous quantitation of four aflatoxins B1, B2, G1, and G2 in peanuts and raisins were performed on ultra-performance liquid chromatography in a combination of fluorescence detector, without derivatization. The advantages were short analysis time, simple sample handling, and reduced solvent consumption. Instrument detection limits of AFB1, AFB2, AFG1, and AFG2 were 0.07, 0.01, 0.1, and 0.008 μg/kg, respectively, lower than those obtained by LCMSMS and HPLC-FLD with derivatization. Two solvent mixtures were chosen for two different matrices whose matrix effect was not negligible (2.81%-8.04% for peanuts and 5.63%-11.43% for raisins). The linear ranges were from 0.2 to 20 μg/L for AFB1 and AFG1 and from 0.05 to 5 μg/L for AFB2 and AFG2. The limits of detection and quantification were 0.025-0.1 and 0.075-0.3 μg/kg for peanuts and raisins, respectively. Recoveries at three other concentrations from 0.75 to 125 μg/kg of total aflatoxins were obtained between 76.5% and 99.8% (with RSD < 6%) following the SANTE 11312/2021. Validation parameters complied with the requirements of ISO/IEC 17025:2017. The extracts and the sample could be stabilized at 4°C and 20°C for 24 h and at -20°C for up to 21 days, respectively. Thus, the study can be used as a standard method for the analysis of Aflatoxins (AFs) in peanut and raisin matrices. Investigation of 350 peanut samples collected at Markets in the central districts of HCM city showed that 28.6% were contaminated with AFB1 from 0.31 up to 554 μg/kg; 13.4% contained AFB2, and 5.7% of AFG1 in the range of 0.4-53 μg/kg and 0.4-9.57 μg/kg, respectively; AFG2 (about 0.6%) was detected from 0.45 to 0.75 μg/kg. Meanwhile, 12.8% exceeded the total aflatoxins limit, and 13.4% exceeded the AFB1 limit. AFs were almost not found in the 350 raisin samples.
摘要：
花生和葡萄干中四种黄曲霉毒素B1，B2，G1和G2的同时定量的优化和验证在超高效液相色谱上结合荧光检测器，没有衍生化。优点是分析时间短，简单的样品处理，减少溶剂消耗。AFB1、AFB2、AFG1和AFG2的仪器检出限分别为0.07、0.01、0.1和0.008μg/kg,分别,低于LCMSMS和衍生化HPLC-FLD获得的。为两种不同的基质选择了两种溶剂混合物，其基质效应不可忽略（花生为2.81％-8.04％，葡萄干为5.63％-11.43％）。AFB1和AFG1的线性范围为0.2至20μg/L，AFB2和AFG2的线性范围为0.05至5μg/L。花生和葡萄干的检出限和定量限为0.025-0.1和0.075-0.3μg/kg，分别。在SANTE11312/2021之后，在其他三个浓度下从0.75至125μg/kg的总黄曲霉毒素的回收率在76.5％至99.8％之间（RSD<6％）。验证参数符合ISO/IEC17025:2017的要求。提取物和样品可以在4°C和20°C下稳定24小时，在-20°C下稳定长达21天，分别。因此，该研究可用作分析花生和葡萄干基质中黄曲霉毒素（AFs）的标准方法。对在HCM市中心区市场收集的350份花生样品的调查显示，28.6％的人被AFB1污染，从0.31到554μg/kg；13.4％的人含有AFB2，5.7％的AFG1在0.4-53μg/kg和0.4-9.57μg/kg的范围内，分别检测到0.45至0.75μg/kg的AFG2（约0.6％）。同时,12.8%超过了黄曲霉毒素总量限值,13.4%超过AFB1限值。在350个葡萄干样品中几乎没有发现AF。
