PDF(Pubmed)
Abstract：
A 25-day-old male common bottlenose dolphin (Tursiops truncatus) died suddenly while swimming at a dolphinarium. The gross examination revealed ulceration on the dorsal and pectoral fins and rostrum. Severe congestion, hemorrhage, and edema were observed in the gastrointestinal tract, liver, mesenteric lymph nodes, lungs, and kidneys. Fibrinosuppurative arthritis of the atlantooccipital joint and extension of fibrin into the spinal canal caused compression of the spinal cord. Histopathological examination revealed tracheitis, fibrinosuppurative bronchopneumonia and enteritis. In the central nervous system, meningeal vessel congestion in the brain, and intraparenchymal hemorrhages with neurodegeneration were observed in the spinal cord. Based on the histopathological findings, representative samples, including lung, liver, mesenteric lymph node, blood obtained from the jugular vein, and fluid sample of the ascites, were inoculated on tryptic soy agar and blood agar for routine bacterial isolation. Each isolated bacterial colony was streaked aseptically onto tryptic soy agar and blood agar for pure culture. After then, polymerase chain reaction (PCR) was performed for further identification of pathogenic microorganisms. PCR identified Escherichia fergusonii, Shewanella haliotis, Enterococcus faecalis, and Staphylococcus schleiferi. E. fergusonii was considered the primary etiologic agent in this case since it was the only species identified in all representative samples. The cause of death in this animal was E. fergusonii sepsis. To the best of our knowledge, this is the first case of neonatal sepsis associated with E. fergusonii infection in a dolphin, and suggests E. fergusonii as an opportunistic pathogen associated with sepsis in dolphins.
摘要：
一只25天大的雄性普通宽吻海豚（Tursiopstruncatus）在海豚馆游泳时突然死亡。大体检查显示背鳍和胸鳍和讲台上有溃疡。严重的充血,出血,在胃肠道中观察到水肿，肝脏,肠系膜淋巴结,肺,还有肾脏.寰枕关节的纤维化脓性关节炎和纤维蛋白向椎管的延伸导致脊髓受压。组织病理学检查显示支气管炎，纤维化脓性支气管肺炎和肠炎。在中枢神经系统中，脑膜血管充血,并观察到脊髓实质内出血伴神经变性。根据组织病理学发现,代表性样本,包括肺,肝脏,肠系膜淋巴结,从颈静脉获得的血液，和腹水的液体样本，接种在胰蛋白酶大豆琼脂和血琼脂上进行常规细菌分离。将每个分离的细菌菌落无菌划线到胰蛋白酶大豆琼脂和血琼脂上用于纯培养。在那之后,进行聚合酶链反应（PCR）以进一步鉴定病原微生物。PCR鉴定出埃希氏菌,HaliotisShewanella,粪肠球菌,和Schleiferi葡萄球菌.在这种情况下，E.Fergusonii被认为是主要的病原体，因为它是所有代表性样品中唯一鉴定的物种。该动物的死亡原因是E.fergusonii败血症。据我们所知,这是第一例与海豚中的E.Fergusonii感染相关的新生儿败血症，并提示Fergusonii是与海豚败血症相关的机会病原体。
