PDF(Pubmed)
Abstract：
UNASSIGNED: TB lymphadenitis is still a problem that needs serious treatment. In Indonesia, it was reported that 53% of TB cases were extrapulmonary tuberculosis, with the most cases being Lymphadenitis TB, 11.6%. In children, 43% of extrapulmonary tuberculosis cases are TB lymphadenitis. Diagnosis is quite difficult; a method of determining the diagnosis and appropriate comprehensive treatment is required in managing TB Lymphadenitis.
UNASSIGNED: In this study, 15 fine needle aspiration biopsy aspirate samples were subjected to molecular examination using the gyrB-polymerase chain reaction method and histopathological observations using the smear method with hematoxylin-eosin staining. Observation of preparations using a microscope with a magnification of 200x.
UNASSIGNED: The histopathological characteristics of the fine needle aspiration biopsy aspirate showed positive results in 4 out of 15 samples, with epithelioid cells arranged in a characteristic granuloma structure, necrotic debris was visible, and cells joined together to form multinucleated giant cells as an inflammatory response to Mycobacterium tuberculosis complex infection. In this study, 6 out of 15 (40%) were detected to be positive in the diagnosis based on molecular detection using a specific target gene gyrB - polymerase chain reaction .
UNASSIGNED: Characteristic features on histopathological examination associated with gyrB - positive polymerase chain reaction on lymphadenitis fine needle aspiration biopsy aspirate samples can be used as a determinant diagnosis of tuberculous lymphadenitis.
UNASSIGNED: In this study, 15 fine needle aspiration biopsy aspirate samples were subjected to molecular examination using the gyrB-polymerase chain reaction method and histopathological observations using the smear method with hematoxylin-eosin staining. Observation of preparations using a microscope with a magnification of 200x.
UNASSIGNED: The histopathological characteristics of the fine needle aspiration biopsy aspirate showed positive results in 4 out of 15 samples, with epithelioid cells arranged in a characteristic granuloma structure, necrotic debris was visible, and cells joined together to form multinucleated giant cells as an inflammatory response to Mycobacterium tuberculosis complex infection. In this study, 6 out of 15 (40%) were detected to be positive in the diagnosis based on molecular detection using a specific target gene gyrB - polymerase chain reaction .
UNASSIGNED: Characteristic features on histopathological examination associated with gyrB - positive polymerase chain reaction on lymphadenitis fine needle aspiration biopsy aspirate samples can be used as a determinant diagnosis of tuberculous lymphadenitis.
摘要：
■TB淋巴结炎仍然是一个需要认真治疗的问题。在印度尼西亚,据报道，53%的结核病病例是肺外结核病，大多数病例是淋巴结炎TB，11.6%。在儿童中，43％的肺外结核病例是结核淋巴结炎。诊断相当困难；在治疗TB淋巴结炎时，需要确定诊断和适当综合治疗的方法。
■在这项研究中，使用gyrB聚合酶链反应方法对15个细针抽吸活检标本进行分子检查，并使用涂片法进行苏木精-伊红染色进行组织病理学观察。使用放大倍数为200x的显微镜观察制剂。
■细针穿刺活检抽吸物的组织病理学特征在15个样本中的4个样本中显示出阳性结果，上皮样细胞排列在特征性肉芽肿结构中，可见坏死碎片，和细胞结合在一起形成多核巨细胞，作为对结核分枝杆菌复合体感染的炎症反应。在这项研究中,15个中的6个（40％）在基于使用特异性靶基因gyrB-聚合酶链反应的分子检测的诊断中被检测为阳性。
■与淋巴结炎细针抽吸活检标本的gyrB阳性聚合酶链反应相关的组织病理学检查的特征可作为结核性淋巴结炎的决定性诊断。
■在这项研究中，使用gyrB聚合酶链反应方法对15个细针抽吸活检标本进行分子检查，并使用涂片法进行苏木精-伊红染色进行组织病理学观察。使用放大倍数为200x的显微镜观察制剂。
■细针穿刺活检抽吸物的组织病理学特征在15个样本中的4个样本中显示出阳性结果，上皮样细胞排列在特征性肉芽肿结构中，可见坏死碎片，和细胞结合在一起形成多核巨细胞，作为对结核分枝杆菌复合体感染的炎症反应。在这项研究中,15个中的6个（40％）在基于使用特异性靶基因gyrB-聚合酶链反应的分子检测的诊断中被检测为阳性。
■与淋巴结炎细针抽吸活检标本的gyrB阳性聚合酶链反应相关的组织病理学检查的特征可作为结核性淋巴结炎的决定性诊断。
