PDF(Pubmed)
Abstract：
UNASSIGNED: Intra-abdominal infection is a complex pathophysiological process involving multiple systems and organs of the body. Abdominal infections complicated by severe sepsis or septic shock have a high mortality rate of 30-50%. Therefore, novel strategies to treat sepsis are urgently needed.
UNASSIGNED: Andrographolide (AD), the main active ingredient of Andrographis paniculata, reportedly exerts beneficial effects on mice with sepsis. However, its exact mechanism of action in attenuating inflammation due to intra-abdominal sepsis remains unclear to date. Hence, this study aimed to examine the therapeutic effects of AD on cecal ligation and puncture (CLP)-induced sepsis and elucidate the underlying mechanisms.
UNASSIGNED: Results showed that AD therapy could significantly improve the 7-day survival rate and alleviate pathological organ injury in mice with CLP. In addition, AD treatment decreased the levels of proinflammatory factors, such as tumor necrosis factor-α and interleukin (IL)-6 in the peritoneal cavity fluid and blood and increased the level of anti-inflammatory factor IL-10 in the peritoneal cavity fluid of mice with CLP. Moreover, bacterial counts in the blood and peritoneal lavage fluid were lower in the mice treated with AD than in those untreated. Mechanistically, AD treatment increased the percentage and phagocytic activity of macrophages in the peritoneal cavity.
UNASSIGNED: These data showed that AD can improve the survival of mice with intra-abdominal sepsis by enhancing bacterial clearance, as evidenced by the increased percentages and phagocytic activity of macrophages in the peritoneal cavity. This study is the first to demonstrate the protective effects of AD against intra-abdominal sepsis.
UNASSIGNED: Andrographolide (AD), the main active ingredient of Andrographis paniculata, reportedly exerts beneficial effects on mice with sepsis. However, its exact mechanism of action in attenuating inflammation due to intra-abdominal sepsis remains unclear to date. Hence, this study aimed to examine the therapeutic effects of AD on cecal ligation and puncture (CLP)-induced sepsis and elucidate the underlying mechanisms.
UNASSIGNED: Results showed that AD therapy could significantly improve the 7-day survival rate and alleviate pathological organ injury in mice with CLP. In addition, AD treatment decreased the levels of proinflammatory factors, such as tumor necrosis factor-α and interleukin (IL)-6 in the peritoneal cavity fluid and blood and increased the level of anti-inflammatory factor IL-10 in the peritoneal cavity fluid of mice with CLP. Moreover, bacterial counts in the blood and peritoneal lavage fluid were lower in the mice treated with AD than in those untreated. Mechanistically, AD treatment increased the percentage and phagocytic activity of macrophages in the peritoneal cavity.
UNASSIGNED: These data showed that AD can improve the survival of mice with intra-abdominal sepsis by enhancing bacterial clearance, as evidenced by the increased percentages and phagocytic activity of macrophages in the peritoneal cavity. This study is the first to demonstrate the protective effects of AD against intra-abdominal sepsis.
摘要：
■腹腔内感染是一个复杂的病理生理过程，涉及身体的多个系统和器官。由严重脓毒症或脓毒性休克并发的腹部感染具有30-50%的高死亡率。因此,迫切需要新的治疗脓毒症的策略.
■穿心莲内酯(AD)，穿心莲的主要活性成分，据报道对败血症小鼠有有益作用。然而,迄今为止,其在减轻腹内脓毒症所致炎症中的确切作用机制尚不清楚.因此，本研究旨在研究AD对盲肠结扎穿孔术(CLP)诱导的脓毒症的治疗作用,并阐明其潜在机制.
■结果表明，AD治疗可明显提高CLP小鼠的7天生存率，减轻病理器官损伤。此外,AD治疗降低了促炎因子的水平，如肿瘤坏死因子-α和白细胞介素(IL)-6在CLP小鼠腹腔液和血液中的表达和抗炎因子IL-10的水平升高。此外，接受AD治疗的小鼠血液和腹腔灌洗液中的细菌计数低于未治疗的小鼠。机械上,AD治疗增加了腹腔中巨噬细胞的百分比和吞噬活性。
■这些数据表明，AD可以通过增强细菌清除来提高腹内脓毒症小鼠的存活率，腹膜腔中巨噬细胞的百分比和吞噬活性增加证明了这一点。这项研究首次证明了AD对腹腔内败血症的保护作用。
■穿心莲内酯(AD)，穿心莲的主要活性成分，据报道对败血症小鼠有有益作用。然而,迄今为止,其在减轻腹内脓毒症所致炎症中的确切作用机制尚不清楚.因此，本研究旨在研究AD对盲肠结扎穿孔术(CLP)诱导的脓毒症的治疗作用,并阐明其潜在机制.
■结果表明，AD治疗可明显提高CLP小鼠的7天生存率，减轻病理器官损伤。此外,AD治疗降低了促炎因子的水平，如肿瘤坏死因子-α和白细胞介素(IL)-6在CLP小鼠腹腔液和血液中的表达和抗炎因子IL-10的水平升高。此外，接受AD治疗的小鼠血液和腹腔灌洗液中的细菌计数低于未治疗的小鼠。机械上,AD治疗增加了腹腔中巨噬细胞的百分比和吞噬活性。
■这些数据表明，AD可以通过增强细菌清除来提高腹内脓毒症小鼠的存活率，腹膜腔中巨噬细胞的百分比和吞噬活性增加证明了这一点。这项研究首次证明了AD对腹腔内败血症的保护作用。
