PDF(Pubmed)
Abstract：
Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery.
摘要：
病毒感染后，病毒抗原与淋巴细胞表面的受体特异性结合，从而激活宿主的适应性免疫。一种表位，抗原最小的结构和功能单位,特异性结合抗体或抗原受体，作为激活适应性免疫的关键位点。表位的复杂性和多样性对于研究和绘制疾病诊断图谱至关重要，疫苗和免疫治疗的设计。定位这些特异性表位已成为免疫学和免疫治疗的热点。最近,表位作图技术已经发展成为多重，随着高通量测序和技术的出现，如噬菌体展示文库和深度突变扫描。这里,我们简要介绍一下原则，优势,以及最新表位作图技术的缺点，包括病毒抗原发现的例子。
