Abstract：
In this study, we present a novel swing-elution-based method to achieve rapid, cost-effective, and easily identifiable blood typing assays. Specifically, the method aims to swing the microfluidic cotton thread-based analytical devices (μCTADs) in PBS solution to effectively elute free red blood cells (RBCs) and allow large agglutinated RBCs to remain to precisely determine the blood type. In order to ensure an easily identifiable blood typing assay, fast swing mode needs to be used, and the elution time is evaluated to be >50 seconds. The created μCTADs have been used to successfully classify ABO and RhD blood types in 56 blood samples. Finally, in order to enhance the convenience and portability of blood typing, a blood-typing chip that utilizes a PBS liquid bridge to effectively elute the free RBCs is designed and fabricated based on the above swing-elution principle. Compared with the traditional wicking-elution methods that rely on the wicking effect to weakly elute the RBCs, our method possesses a stronger elution effect to remove the free RBCs inside the inter-fiber gaps or adhered to the fiber surface, resulting in effectively enhancing the identifiability of the elution results and minimizing user interpretation error. Given the simplicity of the blood typing method, we believe that our blood typing method has great potential to be widely applied in resource-limited and developing regions.
摘要：
在这项研究中,我们提出了一种新的基于摆动洗脱的方法，以实现快速，成本效益高,和易于识别的血液分型测定。具体来说,该方法旨在在PBS溶液中摆动基于棉线的微流控分析设备（μCTAD），以有效洗脱游离红细胞（RBC），并允许大量凝集的RBC保留以精确确定血型。为了确保易于识别的血型检测，需要使用快速摆动模式，和洗脱时间被评估为>50秒。创建的μCTAD已用于成功分类56个血液样本中的ABO和RhD血型。最后,为了提高血液分型的便捷性和便携性，基于上述摆动洗脱原理,设计并制造了利用PBS液桥有效洗脱游离红细胞的血型芯片。与依靠芯吸效应弱洗脱红细胞的传统芯吸洗脱方法相比，我们的方法具有更强的洗脱效果，以去除纤维间间隙内的游离红细胞或粘附在纤维表面，从而有效地增强洗脱结果的可识别性和最小化用户解释错误。鉴于血液分型方法的简单性，我们相信我们的血液分型方法在资源有限和发展中地区具有广泛的应用潜力。
