Abstract：
Endometriosis is an immune chronic inflammatory disease, and there are currently no more effective drugs for treating endometriosis due to its unknown etiology. Salbutamol is a β2-adrenergic receptor (β2AR) agonist commonly used to treat asthma by selectively activating β2 receptors on airway smooth muscle and leukocytes, exerting bronchial dilation and synergistic anti-inflammatory effects. In recent years, β2AR agonists have been used in endometriosis studies, and we speculate that salbutamol may have a therapeutic effect on endometriosis. The purpose of this research was to explore the therapeutic effect of salbutamol on endometriosis mice. The mouse endometriosis model was established and treated with different doses of salbutamol. Endometrial lesions were harvested for pathological diagnosis, immunohistochemistry (IHC), Masson staining, and toluidine blue analysis. We found that the number and size of endometriotic lesions were all significantly decreased after 3 weeks of treatment with different doses of salbutamol on endometriosis model mice (P < 0.05). After Salbutamol treatment, the amount of mast cells (toluidine blue) and macrophages (F4/80) in the lesions as well as the expressions of interleukin (IL)-1β, tumor necrosis factor (TNF)-ɑ, platelet-derived growth factor subunit B (PDGFB), CD31, transforming growth factor (TGF)-β, Masson staining, BCL2, TUBB3, substance P (SP), and nerve growth factor (NGF) were significantly reduced (P < 0.05). These results suggested that salbutamol could effectively treat endometriosis in mice by reducing immune inflammatory cells and factors, angiogenesis, and fibrosis, increasing apoptosis of endometriotic lesions, and decreasing neurogenesis.
摘要：
子宫内膜异位症是一种免疫性慢性炎症性疾病,由于病因不明，目前尚无更有效的治疗子宫内膜异位症的药物。沙丁胺醇是一种β2-肾上腺素能受体（β2AR）激动剂，通常用于通过选择性激活气道平滑肌和白细胞上的β2受体来治疗哮喘，发挥支气管扩张和协同抗炎作用。近年来,β2AR激动剂已用于子宫内膜异位症研究，我们推测沙丁胺醇可能对子宫内膜异位症有治疗作用。目的探讨沙丁胺醇对子宫内膜异位症小鼠的治疗作用。建立小鼠子宫内膜异位症模型,用不同剂量的沙丁胺醇处理。收集子宫内膜病变进行病理诊断，免疫组织化学（IHC），Masson染色,和甲苯胺蓝分析。我们发现，不同剂量沙丁胺醇治疗子宫内膜异位症模型小鼠3周后，子宫内膜异位症病灶的数量和大小均明显减少（P<0.05）。沙丁胺醇治疗后,肥大细胞(甲苯胺蓝)和巨噬细胞(F4/80)的数量以及白细胞介素(IL)-1β的表达,肿瘤坏死因子(TNF)-α,血小板衍生生长因子亚基B(PDGFB),CD31,转化生长因子(TGF)-β,Masson染色,BCL2、TUBB3、P物质(SP)、神经生长因子(NGF)显著降低(P<0.05)。提示沙丁胺醇可通过减少免疫炎症细胞和因子,有效治疗小鼠子宫内膜异位症,血管生成,和纤维化，增加子宫内膜异位病变的细胞凋亡，减少神经发生。
