PDF(Pubmed)
Abstract：
Nonalcoholic fatty liver disease (NAFLD) is highly prevalent in people living with HIV (PLWH) and the expression of some microRNAs could be useful as biomarkers for the diagnosis of NAFLD. The aim of this study was to identify patterns of differential expression of microRNAs in PLWH and assess their diagnostic value for NALFD.
A discovery case-control study with PLWH was carried out. The expression of miRNAs was determined using HTG EdgeSeq technology. Cases were defined as patients with severe NAFLD and controls as patients without NAFLD, characterized using the controlled attenuation parameter (CAP). Cases and controls were matched 1:1 for age, sex, BMI, CD4+ lymphocyte count, active HCV infection, and ART regimen.
Serum 2,083 simultaneous microRNA transcripts were analyzed using HTG technology and compared between cases and controls. Forty-five patients, 23 cases, and 22 controls were included in the study. In the analysis of the expression pattern of the 2,083 microRNAs, no differential expression patterns were found between both groups of patients included in the study.
Analysis of the microRNA transcriptome profile of nonobese PLWH with severe NAFLD did not appear to differ from that of patients without NAFLD. Thus, microRNA might not serve as a proper biomarker for predicting severe NALFD in this population.
A discovery case-control study with PLWH was carried out. The expression of miRNAs was determined using HTG EdgeSeq technology. Cases were defined as patients with severe NAFLD and controls as patients without NAFLD, characterized using the controlled attenuation parameter (CAP). Cases and controls were matched 1:1 for age, sex, BMI, CD4+ lymphocyte count, active HCV infection, and ART regimen.
Serum 2,083 simultaneous microRNA transcripts were analyzed using HTG technology and compared between cases and controls. Forty-five patients, 23 cases, and 22 controls were included in the study. In the analysis of the expression pattern of the 2,083 microRNAs, no differential expression patterns were found between both groups of patients included in the study.
Analysis of the microRNA transcriptome profile of nonobese PLWH with severe NAFLD did not appear to differ from that of patients without NAFLD. Thus, microRNA might not serve as a proper biomarker for predicting severe NALFD in this population.
摘要：
■非酒精性脂肪性肝病（NAFLD）在HIV（PLWH）感染者中非常普遍，一些microRNA的表达可作为诊断NAFLD的生物标志物。这项研究的目的是确定PLWH中microRNA的差异表达模式，并评估其对NALFD的诊断价值。
■用PLWH进行发现病例对照研究。使用HTGEdgeSeq技术确定miRNA的表达。病例定义为重度NAFLD患者，对照组定义为无NAFLD患者。使用受控衰减参数(CAP)表征。病例和对照组的年龄为1：1，性别,BMI,CD4+淋巴细胞计数，活动性HCV感染，和ART方案。
■使用HTG技术分析血清2,083个同时的microRNA转录本，并在病例和对照之间进行比较。45名患者,23例,22名对照纳入研究.在2,083个microRNAs的表达模式分析中，在纳入研究的两组患者之间未发现差异表达模式.
■对重度NAFLD的非肥胖型PLWH的microRNA转录组分析与无NAFLD的患者没有差异。因此，microRNA可能不能作为预测该人群中严重NALFD的适当生物标志物。
■用PLWH进行发现病例对照研究。使用HTGEdgeSeq技术确定miRNA的表达。病例定义为重度NAFLD患者，对照组定义为无NAFLD患者。使用受控衰减参数(CAP)表征。病例和对照组的年龄为1：1，性别,BMI,CD4+淋巴细胞计数，活动性HCV感染，和ART方案。
■使用HTG技术分析血清2,083个同时的microRNA转录本，并在病例和对照之间进行比较。45名患者,23例,22名对照纳入研究.在2,083个microRNAs的表达模式分析中，在纳入研究的两组患者之间未发现差异表达模式.
■对重度NAFLD的非肥胖型PLWH的microRNA转录组分析与无NAFLD的患者没有差异。因此，microRNA可能不能作为预测该人群中严重NALFD的适当生物标志物。
