Abstract：
Investigation of Escherichia coli under electromagnetic fields is of significance in human studies owing to its short doubling time and human-like DNA mechanisms. The present review aims to systematically evaluate the literature to conclude causality between 0 and 300 GHz electromagnetic fields and biological effects in E. coli. To that end, the OHAT methodology and risk of bias tool were employed. Exponentially growing cells exposed for over 30 min at temperatures up to 3 7 ∘ C $3{7}^{\\circ }\\,{\\rm{C}}$ with fluctuations below 1 ∘ C ${1}^{\\circ }\\,{\\rm{C}}$ were included from the Web-of-Knowledge, PubMed, or EMF-Portal databases. Out of 904 records identified, 25 articles satisfied the selection criteria, with four excluded during internal validation. These articles examined cell growth (11 studies), morphology (three studies), and gene regulation (11 studies). Most experiments (85%) in the included studies focused on the extremely low-frequency (ELF) range, with 60% specifically at 50 Hz. Changes in growth rate were observed in 74% of ELF experiments and 71% of radio frequency (RF) experiments. Additionally, 80% of ELF experiments showed morphology changes, while gene expression changes were seen in 33% (ELF) and 50% (RF) experiments. Due to the limited number of studies, especially in the intermediate frequency and RF ranges, establishing correlations between EMF exposure and biological effects on E. coli is not possible.
摘要：
由于其短的倍增时间和类似人的DNA机制，在电磁场下研究大肠杆菌在人体研究中具有重要意义。本综述旨在系统地评估文献，以总结0至300GHz电磁场与大肠杆菌生物学效应之间的因果关系。为此,采用OHAT方法和偏倚风险工具.在高达37°C$3{7}^{\\circ}\\的温度下暴露30分钟以上的指数生长细胞，{\\rm{C}}$,波动低于1○C${1}^{\\circ}\\,{\\rm{C}}$包含在Web-of-Knowledge中,PubMed,或EMF-Portal数据库。在确认的904条记录中,25篇文章满足选择标准，在内部验证过程中排除了四个。这些文章检查了细胞生长（11项研究），形态学（三项研究），和基因调控（11项研究）。纳入研究中的大多数实验（85％）都集中在极低频（ELF）范围内，60％，特别是在50Hz。在74％的ELF实验和71％的射频（RF）实验中观察到生长速率的变化。此外,80％的ELF实验显示形态变化，而基因表达变化在33%(ELF)和50%(RF)实验中可见。由于研究数量有限，特别是在中频和射频范围内，建立EMF暴露与大肠杆菌生物学效应之间的相关性是不可能的。
