Abstract：
To explore the resource components and availability of different parts of Panax quinquefolium in Shandong province, the paper employed the non-targeted metabolomics technology based on ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) to analyze the metabolites and their metabolic pathways in the root, fibril, stem, and leaf of P. quinquefolium. The content of seven ginsenosides and polysaccharides in different parts was determined by high performance liquid chromatography(HPLC) and ultraviolet-visible spectrophotometry(UV-Vis). The results showed that the metabolites were mainly sugars, glycosides, organic acids, amino acids and their derivatives, terpenoids, etc. The total abundance of metabolites followed the trend of leaf > root > fibril > stem. Most of the differential metabolites were concentrated in phenylpropane biosynthesis, flavonoid biosynthesis, citric acid cycle, and amino acid biosynthesis. The leaf contained high levels of sugars, glycosides, amino acids and their derivatives, and flavonoids; the root was rich in terpenoids, volatile oils, vitamins, and lignin; the fibril contained rich organic acids; and the stem had high content of nucleotides and their derivatives. The content of ginsenosides Re and Rb_1 was significantly higher in the root; the content of ginsenosides Rg_1, Rg_2, Rd, F_(11), and polysaccharide was significantly higher in the leaf; and the content of ginsenoside Rb_2 was significantly higher in the stem. We analyzed the resource components and availability of different parts of P. quinquefolium, aiming to provide basic information for the comprehensive development and utilization of P. quinquefolium resources in Shandong province.
摘要：
探讨山东省不同部位西洋参资源组成及可利用性,采用基于超高效液相色谱-串联质谱(UPLC-MS/MS)的非靶向代谢组学技术,原纤维,茎,和西洋参的叶。采用高效液相色谱法(HPLC)和紫外-可见分光光度法(UV-Vis)测定不同部位中7种人参皂苷和多糖的含量。结果表明,代谢产物主要为糖类,糖苷，有机酸,氨基酸及其衍生物，萜类化合物,等。代谢物的总丰度跟随叶的趋势。根和gt;原纤维和gt;茎。大多数差异代谢物集中在苯丙烷生物合成中，类黄酮生物合成，柠檬酸循环，和氨基酸生物合成。叶子含有大量的糖，糖苷，氨基酸及其衍生物，和类黄酮；根部富含萜类化合物，挥发油，维生素,和木质素；原纤维含有丰富的有机酸；茎中含有高含量的核苷酸及其衍生物。人参皂苷Re和Rb_1含量在根中显著升高;人参皂苷Rg_1、Rg_2、Rd、F_(11),且叶中多糖含量明显较高；茎中人参皂苷Rb_2含量明显较高。我们分析了西洋参不同部位的资源成分和可用性，旨在为山东省西洋参资源的综合开发利用提供基础资料。
