Abstract：
Prosthogonimiasis poses a threat to the reproductive system of poultry and wild birds, which are the definitive hosts of the parasite causing this disease. However, the parasite infection of the second intermediate host (dragonfly), the primary vector of this pathogen, is rarely reported. In this study, the prevalence of Prosthogonimus infection in dragonflies was investigated from June 2019 to October 2022 in Heilongjiang Province, northeast China. The species of metacercariae isolated from dragonfly were identified by morphological characteristics, molecular biology techniques, and animal infection experiments. The results showed that 11 species of dragonflies and one damselfly were identified and among six of the dragonflies infected by Prosthogonimus metacercariae, Sympetrum depressiusculum (28.53%) had the highest infection rate among all positive dragonflies, followed by Sympetrum vulgatum (27.86%) and Sympetrum frequens (20.99%), which are preferred hosts, and the total prevalence was 20.39% (2061/10,110) in Heilongjiang Province. Three species of Prosthogoniumus metacercariae were isolated, including Prosthogonimus cuneatus, Prosthogonimus pullucidus, and Prosthogonimus sp., among which P. cuneatus was the dominant species in dragonflies in Heilongjiang Province. This is the first report on the prevalence of Prosthogonimus in dragonflies in China, which provides baseline data for the control of prosthogonimiasis in Heilongjiang Province and a reference for the prevention of prosthogonimiasis in other areas of China.
摘要：
原虫病对家禽和野生鸟类的生殖系统构成威胁,是导致这种疾病的寄生虫的最终宿主。然而,第二中间宿主（蜻蜓）的寄生虫感染，这种病原体的主要媒介，很少报道。在这项研究中,2019年6月至2022年10月，对黑龙江省蜻蜓中的Prothogonimus感染进行了调查，中国东北。通过形态特征鉴定了从蜻蜓中分离出的尾虫的种类，分子生物学技术，和动物感染实验。结果表明，已鉴定出11种蜻蜓和1种豆娘，其中6种被原虫感染的蜻蜓中，在所有阳性蜻蜓中，抑郁症状(28.53%)感染率最高，其次是Sympetrumvulgatum(27.86%)和Sympetrumfrequences(20.99%)，它们是首选主机，黑龙江省总患病率为20.39%(2061/10,110)。分离出了3种原虫，包括假人鱼,Prosthogonimuspullucidus,和Prosthogonimussp.,其中尖杉是黑龙江省蜻蜓的优势种。这是在中国首次报道了蜻蜓中的侧柏,本研究为黑龙江省预防假单胞菌病提供了基线数据,也为我国其他地区预防假单胞菌病提供了参考。
