PDF(Pubmed)
Abstract：
Multiphoton microscopy is a powerful imaging tool for biomedical applications. A variety of techniques and respective benefits exist for multiphoton microscopy, but an enhanced resolution is especially desired. Additionally multiphoton microscopy requires ultrafast pulses for excitation, so optimization of the pulse duration at the sample is critical for strong signals.
We aim to perform enhanced resolution imaging that is robust to scattering using a structured illumination technique while also providing a rapid and easily repeatable means to optimize group delay dispersion (GDD) compensation through to the sample.
Spatial frequency modulation imaging (SPIFI) is used in two domains: the spatial domain (SD) and the wavelength domain (WD). The WD-SPIFI system is an in-line tool enabling GDD optimization that considers all material through to the sample. The SD-SPIFI system follows and enables enhanced resolution imaging.
The WD-SPIFI dispersion optimization performance is confirmed with independent pulse characterization, enabling rapid optimization of pulses for imaging with the SD-SPIFI system. The SD-SPIFI system demonstrates enhanced resolution imaging without the use of photon counting enabled by signal to noise improvements due to the WD-SPIFI system.
Implementing SPIFI in-line in two domains enables full-path dispersion compensation optimization through to the sample for enhanced resolution multiphoton microscopy.
We aim to perform enhanced resolution imaging that is robust to scattering using a structured illumination technique while also providing a rapid and easily repeatable means to optimize group delay dispersion (GDD) compensation through to the sample.
Spatial frequency modulation imaging (SPIFI) is used in two domains: the spatial domain (SD) and the wavelength domain (WD). The WD-SPIFI system is an in-line tool enabling GDD optimization that considers all material through to the sample. The SD-SPIFI system follows and enables enhanced resolution imaging.
The WD-SPIFI dispersion optimization performance is confirmed with independent pulse characterization, enabling rapid optimization of pulses for imaging with the SD-SPIFI system. The SD-SPIFI system demonstrates enhanced resolution imaging without the use of photon counting enabled by signal to noise improvements due to the WD-SPIFI system.
Implementing SPIFI in-line in two domains enables full-path dispersion compensation optimization through to the sample for enhanced resolution multiphoton microscopy.
摘要：
■多光子显微镜是生物医学应用的强大成像工具。多光子显微镜存在各种技术和各自的好处，但是特别需要增强的分辨率。此外，多光子显微镜需要超快脉冲激发，因此，优化样品的脉冲持续时间对于强信号至关重要。
■我们的目标是使用结构化照明技术执行增强分辨率的成像，该成像对散射具有鲁棒性，同时还提供快速且易于重复的方法来优化对样品的群延迟色散(GDD)补偿。
■空间频率调制成像（SPFI）用于两个域：空间域（SD）和波长域（WD）。WD-SPFI系统是一种在线工具，可实现GDD优化，考虑到样品的所有材料。SD-SPFI系统跟随并实现增强的分辨率成像。
■通过独立的脉冲表征确认了WD-SPFI色散优化性能，使脉冲的快速优化成像与SD-SPFI系统。SD-SPFI系统展示了增强的分辨率成像，而无需使用由于WD-SPFI系统导致的信噪比改善而启用的光子计数。
■在两个域中实施SPFI，可实现对样品的全路径色散补偿优化，以实现增强分辨率的多光子显微镜。
■我们的目标是使用结构化照明技术执行增强分辨率的成像，该成像对散射具有鲁棒性，同时还提供快速且易于重复的方法来优化对样品的群延迟色散(GDD)补偿。
■空间频率调制成像（SPFI）用于两个域：空间域（SD）和波长域（WD）。WD-SPFI系统是一种在线工具，可实现GDD优化，考虑到样品的所有材料。SD-SPFI系统跟随并实现增强的分辨率成像。
■通过独立的脉冲表征确认了WD-SPFI色散优化性能，使脉冲的快速优化成像与SD-SPFI系统。SD-SPFI系统展示了增强的分辨率成像，而无需使用由于WD-SPFI系统导致的信噪比改善而启用的光子计数。
■在两个域中实施SPFI，可实现对样品的全路径色散补偿优化，以实现增强分辨率的多光子显微镜。
