UNASSIGNED: We collected samples of excess blood, serum, and cerebrospinal fluid (CSF) from 6 patients with ocular syphilis treated in 2013-2016. DNA was extracted, purified, and then analyzed using an enhanced molecular typing method including sequence analysis of tp0548, number of repeats in the arp gene, and restriction fragment length polymorphism of the tpr gene.
UNASSIGNED: Molecular strain typing based on tp0548 gene sequence analysis revealed two cases of type F and two cases of type G in 3 of 6 (50%) cases with CSF samples, 1 of which was obtained after starting antibiotics. In a patient with 2 distinct episodes, the same tp0548 type (type G) was identified in both episodes using different sample types (CSF, whole blood). Serum samples were available in 6 cases, but none were successfully typed with any of the methods. Amplification of the tpr and arp genes was unsuccessful in all cases. Overall, strain types were identified in 4 of the 7 episodes.
UNASSIGNED: Treponema pallidum strain types F and G were detected in CSF or whole blood in 4 of 7 episodes in this series. We demonstrate moderate sensitivity of strain typing in ocular syphilis using non-ocular clinical specimens.
■我们采集了多余的血液样本,血清,和脑脊液(CSF)来自2013-2016年治疗的6例眼梅毒患者。提取DNA,纯化,然后使用增强的分子分型方法进行分析,包括tp0548的序列分析,arp基因中的重复数,和tpr基因的限制性片段长度多态性。
■基于tp0548基因序列分析的分子菌株分型显示,在6例(50%)CSF样本中的3例中,有2例F型和2例G型,其中1是在开始抗生素后获得的。在有2次不同发作的患者中,使用不同的样本类型(CSF,全血)。6例有血清样本,但没有成功键入任何方法。tpr和arp基因的扩增在所有情况下都不成功。总的来说,在7例中的4例中确定了菌株类型。
■在该系列的7次发作中,有4次在CSF或全血中检测到F和G型梅毒螺旋体菌株。我们证明了使用非眼部临床标本对眼部梅毒进行应变分型的中等敏感性。