Abstract：
Six psychrotolerant, Gram-stain-negative, aerobic bacterial strains, designated as LB1P51T, LB2P87T, LB2P84, LB3P48, LB3R18 and XS2P67, were isolated from glaciers on the Tibetan Plateau, PR China. The results of 16S rRNA gene analysis confirmed their classification within the genus Flavobacterium. Strain LB2P87T displayed the highest sequence similarity to Flavobacterium sinopsychrotolerans 0533T (98.18 %), while strain LB1P51T exhibited the highest sequence similarity to Flavobacterium glaciei CGMCC 1.5380T (98.15 %). Strains LB2P87T and LB1P51T had genome sizes of 3.8 and 3.9 Mb, respectively, with DNA G+C contents of 34.2 and 34.1 %, respectively. Pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) calculations revealed that these strains represented two distinct species within the genus Flavobacterium. The results of phylogenomic analysis using 606 core genes indicated that the six strains formed a distinct clade and were most closely related to F. glaciei CGMCC 1.5380T. The ANI and dDDH values between the two species and other members of the genus Flavobacterium were below 90.3 and 40.1 %, respectively. Genome relatedness, the results of phylogenomic analysis and phenotypic characteristics collectively support the proposal of two novel species of the genus Flavobacterium: Flavobacterium algoritolerans sp. nov. (LB1P51T = CGMCC 1.11237T = NBRC 114813T) and Flavobacterium yafengii sp. nov. (LB2P87T = CGMCC 1.11249T = NBRC 114814T).
摘要：
六个精神耐受者，革兰氏染色阴性，好氧细菌菌株，指定为LB1P51T，LB2P87T,LB2P84，LB3P48，LB3R18和XS2P67是从青藏高原的冰川中分离出来的，公关中国。16SrRNA基因分析的结果证实了它们在黄杆菌属中的分类。菌株LB2P87T显示出最高的序列相似性与黄体黄杆菌0533T（98.18％），而菌株LB1P51T与冰川黄杆菌CGMCC1.5380T的序列相似性最高(98.15%)。菌株LB2P87T和LB1P51T的基因组大小为3.8和3.9Mb，分别,DNAG+C含量分别为34.2和34.1%，分别。成对平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）计算表明，这些菌株代表黄杆菌属中的两个不同物种。使用606个核心基因进行系统基因组分析的结果表明，这6个菌株形成了一个不同的进化枝，并且与冰川F.CGMCC1.5380T。两物种与黄杆菌属其他成员之间的ANI和dDDH值分别低于90.3和40.1%,分别。基因组相关性，系统基因组分析和表型特征的结果共同支持了黄杆菌属的两个新物种的提议：黄杆菌属。11月。（LB1P51T=CGMCC1.11237T=NBRC114813T）和雅芬基黄杆菌。11月。（LB2P87T=CGMCC1.11249T=NBRC114814T）。
