Abstract：
Kidney stones have been associated with an increased risk of chronic kidney diseases, end-stage renal failure. This study is devoted to isolate nanobacteria from patients with active urolithiasis and investigate the in vitro and in vivo antinanobacterial activity of some antibiotics alone or in combination with extracts of irradiated herbs from certain medicinal plants. Nanobacteria were detected using scanning (SEM) and transmission (TEM) electron microscopy, protein electrophoresis (SDS-PAGE) and DNA profile. The antimicrobial susceptibility of some biofilm-producing nanobacterial isolates was evaluated. The effect of medicinal plant extracts on growth was tested. A combination treatment between the most potent extracts and antibiotics was tested on biofilm production, protein profile, release of 260 nm absorbing material, protein content, and ultrastructure of the strongest biofilm producers. In vivo study of nanobacteria and its treatment by the most potent agents was evaluated on male rats. Renal function was measured in serum; histological examination and oxidative stress parameters were determined in kidney tissues. Results showed that streptomycin, trimethoprim/sulfamethoxazole, doxycycline, and water extracts of irradiated khella at 6 kGy had antinanobacterial activity. Meanwhile, the synergistic effect of the aqueous extract of irradiated Khella and doxycycline showed higher inhibition activity on microbial growth and biofilm production. They affected dramatically the strength of its cell membrane and subsequently its ultrastructure. Moreover, these results are confirmed by ameliorations in renal function and histological alterations. It could be concluded that the combination of DO and an aqueous extract of irradiated khella has an antinephrotoxic effect against nanobacteria-induced renal toxicity.
摘要：
肾结石与慢性肾脏疾病的风险增加有关，终末期肾衰竭。这项研究致力于从活动性尿石症患者中分离纳米细菌，并研究某些抗生素单独或与某些药用植物的辐照草药提取物联合使用的体外和体内抗细菌活性。使用扫描（SEM）和透射（TEM）电子显微镜检测纳米细菌，蛋白质电泳（SDS-PAGE）和DNA图谱。评估了一些产生生物膜的纳米细菌分离株的抗微生物敏感性。测试了药用植物提取物对生长的影响。在生物膜生产中测试了最有效的提取物和抗生素之间的组合处理，蛋白质谱，释放260nm吸收材料，蛋白质含量，和最强大的生物膜生产者的超微结构。在雄性大鼠上评估了纳米细菌的体内研究及其用最有效的药物进行的治疗。在血清中测量肾功能；在肾组织中确定组织学检查和氧化应激参数。结果表明，链霉素，甲氧苄啶/磺胺甲恶唑，多西环素,在6kGy的辐照下，荷拉的水提取物具有抗细菌活性。同时,辐照的Khella和强力霉素的水提取物对微生物生长和生物膜产生具有更高的抑制作用。它们显着影响其细胞膜的强度以及随后的超微结构。此外，肾功能和组织学改变的改善证实了这些结果。可以得出结论，DO和辐照的khella的水提取物的组合具有抗纳米细菌诱导的肾毒性的作用。
