Abstract：
UNASSIGNED: Neutrophils continuously migrate into the oral cavity from various sources like gingival crevicular fluid and saliva both in health and in inflammation. The migration of the neutrophils into the various tissues and into the oral cavity occurs when the host microbial interplay tips the balance favoring the initiation of the inflammatory and immune reactions which depending on the amount of the microbial load results in the development of acute and chronic infections in the susceptible host.
UNASSIGNED: The present study was designed to quantify and compare the oral salivary neutrophil levels in patients with gingivitis and chronic and aggressive periodontitis as well as in healthy controls, before and after scaling and root planing (SRP) and to compare the difference within the selected study groups.
UNASSIGNED: Forty subjects were classified into four groups, that is, healthy controls, gingivitis, and chronic and aggressive periodontitis. Oral rinse samples were collected using Hank\'s balanced salt solution from each patient before and after phase I periodontal therapy. Cells in the rinse samples were stained with Acridine orange, and neutrophil counts were carried out using a fluorescence microscope and a hemocytometer.
UNASSIGNED: Baseline oral salivary neutrophil levels were maximum in the chronic periodontitis group followed by the aggressive group and then the gingivitis group. Oral salivary neutrophil levels also positively correlated to probing pocket depth, plaque index, calculus index, and gingival index in all four study groups. Maximum reduction in the oral salivary neutrophil levels after phase I periodontal therapy was seen in the gingivitis group.
UNASSIGNED: From our study, we conclude that the oral salivary neutrophil levels decreased significantly after SRP. Estimation of changes in the oral salivary neutrophil levels has the potential to aid in monitoring treatment outcomes. Thus, it suggests that it could be used as a simple, noninvasive laboratory technique to monitor the periodontal status and disease progression.
UNASSIGNED: The present study was designed to quantify and compare the oral salivary neutrophil levels in patients with gingivitis and chronic and aggressive periodontitis as well as in healthy controls, before and after scaling and root planing (SRP) and to compare the difference within the selected study groups.
UNASSIGNED: Forty subjects were classified into four groups, that is, healthy controls, gingivitis, and chronic and aggressive periodontitis. Oral rinse samples were collected using Hank\'s balanced salt solution from each patient before and after phase I periodontal therapy. Cells in the rinse samples were stained with Acridine orange, and neutrophil counts were carried out using a fluorescence microscope and a hemocytometer.
UNASSIGNED: Baseline oral salivary neutrophil levels were maximum in the chronic periodontitis group followed by the aggressive group and then the gingivitis group. Oral salivary neutrophil levels also positively correlated to probing pocket depth, plaque index, calculus index, and gingival index in all four study groups. Maximum reduction in the oral salivary neutrophil levels after phase I periodontal therapy was seen in the gingivitis group.
UNASSIGNED: From our study, we conclude that the oral salivary neutrophil levels decreased significantly after SRP. Estimation of changes in the oral salivary neutrophil levels has the potential to aid in monitoring treatment outcomes. Thus, it suggests that it could be used as a simple, noninvasive laboratory technique to monitor the periodontal status and disease progression.
摘要：
■中性粒细胞在健康和炎症中从各种来源如龈沟液和唾液连续迁移到口腔中。当宿主微生物相互作用提示有利于引发炎症和免疫反应的平衡时,嗜中性粒细胞迁移到各种组织中并进入口腔中,这取决于微生物负荷的量,导致在易感宿主中发展急性和慢性感染。
本研究旨在量化和比较牙龈炎、慢性和侵袭性牙周炎患者以及健康对照患者的口腔唾液中性粒细胞水平。在缩放和根平整（SRP）之前和之后，并比较所选研究组内的差异。
■将40名受试者分为四组，也就是说,健康的控制,牙龈炎,和慢性侵袭性牙周炎.在I期牙周治疗之前和之后，使用Hank\的平衡盐溶液从每位患者收集口腔冲洗样品。冲洗样品中的细胞用吖啶橙染色，使用荧光显微镜和血细胞计数器进行中性粒细胞计数。
■基线口腔唾液中性粒细胞水平在慢性牙周炎组最高，其次是侵袭性组，然后是牙龈炎组。口腔唾液中性粒细胞水平也与探查袋深度呈正相关，菌斑指数,微积分指数，所有四个研究组的牙龈指数。在牙龈炎组中，经过I期牙周治疗后，口腔唾液中性粒细胞水平最大程度地降低。
■从我们的研究来看，我们得出的结论是，SRP后口服唾液中性粒细胞水平显着降低。评估口腔唾液中性粒细胞水平的变化可能有助于监测治疗结果。因此，这表明它可以作为一个简单的，无创实验室技术监测牙周状况和疾病进展。
本研究旨在量化和比较牙龈炎、慢性和侵袭性牙周炎患者以及健康对照患者的口腔唾液中性粒细胞水平。在缩放和根平整（SRP）之前和之后，并比较所选研究组内的差异。
■将40名受试者分为四组，也就是说,健康的控制,牙龈炎,和慢性侵袭性牙周炎.在I期牙周治疗之前和之后，使用Hank\的平衡盐溶液从每位患者收集口腔冲洗样品。冲洗样品中的细胞用吖啶橙染色，使用荧光显微镜和血细胞计数器进行中性粒细胞计数。
■基线口腔唾液中性粒细胞水平在慢性牙周炎组最高，其次是侵袭性组，然后是牙龈炎组。口腔唾液中性粒细胞水平也与探查袋深度呈正相关，菌斑指数,微积分指数，所有四个研究组的牙龈指数。在牙龈炎组中，经过I期牙周治疗后，口腔唾液中性粒细胞水平最大程度地降低。
■从我们的研究来看，我们得出的结论是，SRP后口服唾液中性粒细胞水平显着降低。评估口腔唾液中性粒细胞水平的变化可能有助于监测治疗结果。因此，这表明它可以作为一个简单的，无创实验室技术监测牙周状况和疾病进展。
