Abstract:
The present study aims to investigate if Cimicifuga racemosa (L.) Nutt extract (CIMI) reduces deleterious effects of dexamethasone (DEXA) in ovaries cultured in vitro. Mouse ovaries were collected and cultured in DMEM+ only or supplemented with 5 ng/mL of CIMI, or 4 ng/mL DEXA, or both CIMI and DEXA. The ovaries were cultured at 37.5°C in 5% CO2 for 6 days. Ovarian morphology, follicular ultrastructure, and the levels of mRNA for Bax, Bcl-2, and Caspase-3 were evaluated. The results showed that DEXA reduced the percentage of morphologically normal follicles, while CIMI prevented the deleterious effects caused by DEXA. In addition, DEXA negatively affected the stromal cellular density, while CIMI prevented these adverse effects. Ovaries cultured with DEXA and CIMI showed similar levels of mRNA for Bax, Bcl-2, and Caspase-3 compared to those cultured in control medium, while ovaries cultured with DEXA had increased expression of the above genes. Additionally, the ultrastructure of the ovaries cultured with CIMI was well preserved. Thus, the extract of CIMI was able to prevent the deleterious effects caused by DEXA on cultured mouse ovaries.
摘要:
本研究旨在调查cimicifugaraceemosa(L.)Nutt提取物(CIMI)降低了体外培养的卵巢中地塞米松(DEXA)的有害作用。收集小鼠卵巢并仅在DMEM中培养或补充5ng/mL的CIMI,或4ng/mLDEXA,或者CIMI和DEXA。将卵巢在37.5°C下在5%CO2中培养6天。卵巢形态学,卵泡超微结构,以及Bax的mRNA水平,评估Bcl-2和Caspase-3。结果表明,DEXA降低了形态正常卵泡的百分比,而CIMI可预防DEXA引起的有害影响。此外,DEXA对基质细胞密度有负面影响,而CIMI预防了这些不良反应。用DEXA和CIMI培养的卵巢显示Bax的mRNA水平相似,与在对照培养基中培养的Bcl-2和Caspase-3相比,而用DEXA培养的卵巢增加了上述基因的表达。此外,CIMI培养的卵巢超微结构保存良好。因此,CIMI的提取物能够防止DEXA对培养的小鼠卵巢造成的有害影响。