Abstract：
Engine oil spills have been associated with a wide range of human health problems. However, little is known about the effects of petroleum hydrocarbon pollution on soil microbial communities. In this study, three samples were collected from oil-polluted soils (OPS), and one control soil (CS) from Taolin town, China, near the old engine\'s scrapes was used. The aims of this study were to conduct metagenomic sequencing and subsequently perform resistome and virulome analysis. We also aimed to validate anti-microbial resistance and virulence genes and anti-bacterial sensitivity profiles among the isolates from oil-polluted soils. The OPS microbial community was dominated by bacterial species compared to the control samples which were dominated by metazoans and other organisms. Secondly, the resistosome and virulome analysis showed that ARGs and virulence factors were higher among OPS microbial communities. Antibiotic susceptibility assay and qPCR analysis for ARGs and virulence factors showed that the oil-polluted soil samples had remarkably enhanced expression of these ARGs and some virulence genes. Our study suggests that oil pollution contributes to shifting microbial communities to more resilient types that could survive the toxicity of oil pollution and subsequently become more resilient in terms of higher resistance and virulence potential.
摘要：
背景技术发动机油泄漏已经与广泛的人类健康问题相关联。然而,石油烃污染对土壤微生物群落的影响知之甚少。在这项研究中,从石油污染土壤(OPS)中收集了三个样本，和一个来自桃林镇的控制土壤(CS)，中国,附近的旧发动机的碎片被使用。本研究的目的是进行宏基因组测序，随后进行抗性组和病毒组分析。我们还旨在验证石油污染土壤中分离株的抗微生物抗性和毒力基因以及抗细菌敏感性谱。与以后生动物和其他生物体为主的对照样品相比，OPS微生物群落以细菌物种为主。其次,抗性小体和病毒组分析表明，OPS微生物群落中ARGs和毒力因子较高。抗生素敏感性测定和对ARGs和毒力因子的qPCR分析表明，石油污染土壤样品中这些ARGs和某些毒力基因的表达显着增强。我们的研究表明，石油污染有助于将微生物群落转移到更具弹性的类型，这些类型可以在石油污染的毒性中幸存下来，随后在更高的抗性和毒力潜力方面变得更具弹性。
