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Abstract：
The aim of this study was to analyze the role of Ezrin in esophageal squamous cell carcinoma (ESCC) and investigate potential therapeutic targets for ESCC by interfering with Ezrin expression. Bioinformatics analysis revealed that Ezrin expression differed significantly among patients with different clinical stage ESCC. Moreover, there was a significant correlation between Ezrin and yes-associated protein/connective tissue growth factor (YAP1/CTGF) levels in esophageal cancer. Sixty paraffin-embedded ESCC tissue samples were examined and Ezrin and YAP1/CTGF levels were determined using immunohistochemistry. The positive expression rates of Ezrin and YAP1/CTGF were significantly lower in adjacent tissues than in ESCC tissues. Furthermore, knockdown of Ezrin expression inhibited colony formation and reduced cell migration and invasion. Compared with control ESCC cells, protein expression levels of YAP1 and CTGF were significantly downregulated in cells with Ezrin knocked down. We conclude that Ezrin may be involved in ESCC progression through the Hippo signaling pathway.
摘要：
这项研究的目的是分析Ezrin在食管鳞状细胞癌（ESCC）中的作用，并通过干扰Ezrin表达来研究ESCC的潜在治疗靶标。生物信息学分析显示Ezrin在不同临床分期ESCC患者中的表达差异显著。此外，食管癌中Ezrin与Yes相关蛋白/结缔组织生长因子（YAP1/CTGF）水平之间存在显着相关性。检查了60个石蜡包埋的ESCC组织样品，并使用免疫组织化学测定了Ezrin和YAP1/CTGF水平。Ezrin和YAP1/CTGF在癌旁组织中的阳性表达率显著低于ESCC组织。此外，Ezrin表达的敲除抑制了集落的形成，并降低了细胞的迁移和侵袭。与对照ESCC细胞相比，在Ezrin敲低的细胞中，YAP1和CTGF的蛋白表达水平显著下调。我们得出结论，Ezrin可能通过Hippo信号通路参与ESCC的进展。
