PDF(Pubmed)
Abstract：
Kaposi\'s sarcoma-associated herpesvirus (KSHV) is a large, oncogenic DNA virus belonging to the gammaherpesvirus subfamily. KSHV has been extensively studied with various high-throughput RNA-sequencing approaches to map the transcription start and end sites, the splice junctions, and the translation initiation sites. Despite these efforts, the comprehensive annotation of the viral transcriptome remains incomplete. In the present study, we generated a long-read sequencing dataset of the lytic and latent KSHV transcriptome using native RNA and direct cDNA sequencing methods. This was supplemented with CAGE sequencing based on a short-read platform. We also utilized datasets from previous publications for our analysis. As a result of this combined approach, we have identified a number of novel viral transcripts and RNA isoforms and have either corroborated or improved the annotation of previously identified viral RNA molecules, thereby notably enhancing our comprehension of the transcriptomic architecture of the KSHV genome. We also evaluated the coding capability of transcripts previously thought to be non-coding, by integrating our data on the viral transcripts with translatomic information from other publications.
摘要：
卡波西肉瘤相关疱疹病毒（KSHV）是一种大型的，属于γ疱疹病毒亚家族的致癌DNA病毒。KSHV已通过各种高通量RNA测序方法进行了广泛研究，以绘制转录起始和结束位点，拼接点，和翻译启动网站。尽管做出了这些努力,病毒转录组的综合注释仍然不完整。在本研究中,我们使用天然RNA和直接cDNA测序方法生成了裂解和潜伏KSHV转录组的长读数测序数据集。这补充了基于短读取平台的CAGE测序。我们还利用以前出版物的数据集来进行分析。由于这种组合方法，我们已经鉴定了许多新的病毒转录物和RNA亚型，并且已经证实或改进了先前鉴定的病毒RNA分子的注释，从而显著增强我们对KSHV基因组转录组结构的理解。我们还评估了以前认为非编码的转录本的编码能力，通过整合我们的病毒转录本数据与其他出版物的翻译信息。
目的：解密KSHV的病毒转录组非常重要，因为我们可以深入了解病毒复制和发病机理的分子机制，这可以帮助开发抗病毒干预的潜在目标。具体来说,这项工作对大量转录重叠的鉴定表明转录机制之间存在全基因组干扰。这一发现表明存在一个新的监管层，可能控制病毒基因的表达。
目的：解密KSHV的病毒转录组非常重要，因为我们可以深入了解病毒复制和发病机理的分子机制，这可以帮助开发抗病毒干预的潜在目标。具体来说,这项工作对大量转录重叠的鉴定表明转录机制之间存在全基因组干扰。这一发现表明存在一个新的监管层，可能控制病毒基因的表达。
