Abstract：
Soil protozoa are an essential part of the terrestrial ecosystem, playing a vital role in the global element cycling and energy flow. However, one research gap is what are the key factors driving their diversity and environmental fates. In this study, we hypothesized that soil texture could affect soil protozoa\'s predation and their interactions with environmental pollutants, and we tested it by using a soil amoeba Dictyostelium discoideum as a model system. We found that soil texture affected amoeba\'s growth and development. In addition, environmental factors cannot explain the variation of amoeba\'s fitness in different soil textures. Soil sandy particles and water content rather than particle size contribute to amoeba\'s fitness. Furthermore, different soil textures induced distinct transcriptional responses to amoebae, especially N-glycan-related and multiple signaling pathways and the expression of key genes (e.g., Ras superfamily, cxgE, trap1). The expression of N-glycan-related pathways, which is positively correlated with amoeba predation, was inhibited in sand soil, decreasing amoeba\'s fitness. Finally, the results showed that soil texture also affects amoeba\'s interaction with environmental pollutants. In conclusion, this study shows that soil physical structures affect amoeba\'s interactions with bacteria and environmental pollutants. SYNOPSIS: Soil texture affects soil protozoa\'s growth and development and their interactions with environmental pollutants.
摘要：
土壤原生动物是陆地生态系统的重要组成部分,在全球元素循环和能量流动中起着至关重要的作用。然而,一个研究差距是什么是驱动他们的多样性和环境命运的关键因素。在这项研究中,我们假设土壤质地可能影响土壤原生动物的捕食及其与环境污染物的相互作用，我们用土壤阿米巴盘基网柄菌作为模型系统进行了测试。我们发现土壤质地影响变形虫的生长发育。此外,环境因素不能解释不同土壤质地变形虫适应度的变化。土壤沙质颗粒和含水量而不是颗粒大小有助于变形虫的健康。此外，不同的土壤质地对变形虫诱导不同的转录反应，特别是N-聚糖相关和多个信号通路以及关键基因的表达(例如，拉斯超家族,cxgE,trap1).N-聚糖相关通路的表达，这与变形虫捕食呈正相关，在沙土中被抑制，变形虫的健康下降。最后,结果表明，土壤质地也影响变形虫与环境污染物的相互作用。总之,这项研究表明，土壤物理结构影响变形虫与细菌和环境污染物的相互作用。简介:土壤质地影响土壤原生动物的生长发育及其与环境污染物的相互作用。
