Abstract：
The COVID-19 pandemic has further intensified plastic pollution due to the escalated use of single-use gloves and masks, consequently leading to the widespread presence of microplastics (MPs) and nanoplastics (NPs) in major rivers and lakes worldwide. Macrobrachium rosenbergii has become an important experimental subject due to its ecological role and environmental sensitivity. In this study, we sought to comprehend the ramifications of NPs on the widely-distributed freshwater prawn, M rosenbergii, by conducting a detailed analysis of its responses to NPs after both 96 h and 30 days of exposure. The transcriptome analysis revealed 918 differentially expressed unigenes (DEGs) after 30 days of NPs exposure (356 upregulated, 562 downregulated) and 2376 DEGs after 96 h of NPs exposure (1541 upregulated, 835 downregulated). The results of DEGs expression indicated that acute NPs exposure enhanced carbohydrate transport and metabolism, fostering chitin and extracellular matrix processes. In contrast, chronic NPs exposure induced nucleolar stress in M. rosenbergii, impeding ribosome development and mRNA maturation while showing no significant changes in glucose metabolism. Our findings underscore the M. rosenbergii distinct coping mechanisms during acute and chronic NPs exposure, elucidating its vital adaptive strategies. These results contribute to our understanding of the ecological implications of NPs pollution and its impact on aquatic animals.
摘要：
由于一次性手套和口罩的使用增加，新冠肺炎疫情进一步加剧了塑料污染，因此导致微塑料（MPs）和纳米塑料（NPs）在全球主要河流和湖泊中的广泛存在。罗氏沼虾由于其生态作用和环境敏感性而成为重要的实验对象。在这项研究中,我们试图理解NPs对广泛分布的淡水虾的影响，罗森贝吉女士,通过详细分析其96小时和30天暴露后对NPs的反应。转录组分析显示，在NPs暴露30天后，918个差异表达的单基因(DEG)(356个上调，562个下调)和2376个DEG在96小时的NPs暴露后(1541个上调，835下调）。DEGs的表达结果表明，急性NPs暴露增强了碳水化合物的运输和代谢，促进几丁质和细胞外基质过程。相比之下,慢性NPs暴露诱导罗森博吉氏杆菌的核仁应激，阻碍核糖体发育和mRNA成熟，而葡萄糖代谢没有显着变化。我们的研究结果强调了急性和慢性NPs暴露期间罗森贝吉不同的应对机制，阐明其重要的适应策略。这些结果有助于我们了解NPs污染的生态含义及其对水生动物的影响。
