PDF(Pubmed)
Abstract：
UNASSIGNED: In the first year of life, DNA methylation (DNAm) patterns are established and are particularly susceptible to exposure-induced changes. Some of these changes may leave lasting effects by persistently altering gene expression or cell type composition or function, contributing to disease.
UNASSIGNED: In this discovery study, we investigated DNAm associations with sensitization to peanut, egg, or cow\'s milk and hypothesized that genes demonstrating DNAm differences in immune cells may play a role in the development of food sensitization.
UNASSIGNED: Infant sensitization (a skin prick test wheal size that is at least 2 mm greater than the negative control) was measured to peanut, egg, and cow\'s milk at age 1 year, and ages of food introduction were reported prospectively. PBMC DNAm was measured in blood samples at 1 year in 144 infants, oversampled for atopy or wheeze. Statistical analysis of Illumina 450k array DNAm data was conducted in R with adjustment for clinical and genetic covariables and a minimum effect size of 1%, false discovery rate of 5%, and medium-confidence false discovery rate threshold of 20%.
UNASSIGNED: There were no DNAm differences between infants with and without peanut, egg, or cow\'s milk sensitization. Borderline significant sites with high effect sizes were enriched for methylation quantitative trait loci, hinting at genetic factors influencing DNAm at these sites. DNAm patterns did not differ by peanut or egg introduction before or after 12 months.
UNASSIGNED: This small pilot study did not show differences in methylation by food sensitization or introduction, but it did demonstrate DNAm patterns linked to genetic variants.
UNASSIGNED: In this discovery study, we investigated DNAm associations with sensitization to peanut, egg, or cow\'s milk and hypothesized that genes demonstrating DNAm differences in immune cells may play a role in the development of food sensitization.
UNASSIGNED: Infant sensitization (a skin prick test wheal size that is at least 2 mm greater than the negative control) was measured to peanut, egg, and cow\'s milk at age 1 year, and ages of food introduction were reported prospectively. PBMC DNAm was measured in blood samples at 1 year in 144 infants, oversampled for atopy or wheeze. Statistical analysis of Illumina 450k array DNAm data was conducted in R with adjustment for clinical and genetic covariables and a minimum effect size of 1%, false discovery rate of 5%, and medium-confidence false discovery rate threshold of 20%.
UNASSIGNED: There were no DNAm differences between infants with and without peanut, egg, or cow\'s milk sensitization. Borderline significant sites with high effect sizes were enriched for methylation quantitative trait loci, hinting at genetic factors influencing DNAm at these sites. DNAm patterns did not differ by peanut or egg introduction before or after 12 months.
UNASSIGNED: This small pilot study did not show differences in methylation by food sensitization or introduction, but it did demonstrate DNAm patterns linked to genetic variants.
摘要：
■在生命的第一年，DNA甲基化（DNAm）模式已建立，并且特别容易受到暴露诱导的变化的影响。这些变化中的一些可能会通过持续改变基因表达或细胞类型组成或功能而留下持久的影响。促成疾病。
■在这项发现研究中，我们调查了DNAm与花生致敏的关系，鸡蛋,或牛奶，并假设证明免疫细胞中DNAm差异的基因可能在食物致敏的发展中起作用。
■对花生测量婴儿致敏（皮肤刺痛试验风团大小至少比阴性对照大2毫米），鸡蛋,和1岁时的牛奶，和食物引入的年龄是前瞻性报道的。在144名婴儿1岁时的血液样本中测量PBMCDNAm，过度采样为特应性或喘息。在R中进行Illumina450k阵列DNAm数据的统计分析，并调整临床和遗传协变量，最小效应大小为1％，错误发现率为5%，中等置信度错误发现率阈值为20%。
■有和没有花生的婴儿之间没有DNAm差异，鸡蛋,或牛奶致敏。具有高效应大小的临界显著位点富集了甲基化数量性状基因座，暗示影响这些位点DNAm的遗传因素。在12个月之前或之后，花生或鸡蛋的引入对DNAm模式没有差异。
■这项小型试点研究未显示食物致敏或引入导致的甲基化差异，但它确实证明了与遗传变异有关的DNAm模式。
■在这项发现研究中，我们调查了DNAm与花生致敏的关系，鸡蛋,或牛奶，并假设证明免疫细胞中DNAm差异的基因可能在食物致敏的发展中起作用。
■对花生测量婴儿致敏（皮肤刺痛试验风团大小至少比阴性对照大2毫米），鸡蛋,和1岁时的牛奶，和食物引入的年龄是前瞻性报道的。在144名婴儿1岁时的血液样本中测量PBMCDNAm，过度采样为特应性或喘息。在R中进行Illumina450k阵列DNAm数据的统计分析，并调整临床和遗传协变量，最小效应大小为1％，错误发现率为5%，中等置信度错误发现率阈值为20%。
■有和没有花生的婴儿之间没有DNAm差异，鸡蛋,或牛奶致敏。具有高效应大小的临界显著位点富集了甲基化数量性状基因座，暗示影响这些位点DNAm的遗传因素。在12个月之前或之后，花生或鸡蛋的引入对DNAm模式没有差异。
■这项小型试点研究未显示食物致敏或引入导致的甲基化差异，但它确实证明了与遗传变异有关的DNAm模式。
