PDF(Pubmed)
Abstract：
Manual dish preparation for IVF in human fertility clinics or animal laboratories heavily relies on embryologists\' experience, which can lead to occupational illness due to long-term and monotonous operation. Therefore, introducing an automated technique to replace traditional methods is crucial for improving working efficiency and reducing work burden for embryologists. In the current study in the mouse, both manual and automated methods were used to prepare IVF or embryo culture dishes. A one-way analysis of variance was conducted to compare several factors, including preparation time, qualified rates, media osmolality of dishes, fertilization rates, and embryonic development to assess the efficiency and potential of automated preparation. The results showed that automation system significantly reduced the required time and increased the efficiencies and qualified rates of dish preparation, especially for embryo culture dishes, without significantly altering medium osmolalities. There were no significant differences between two preparations in fertilization rates and embryo development in mice. Thus, automated dish preparation can improve working efficiency and qualified rates while maintaining fertilization rates and subsequent embryonic development without compromising osmolality stability of medium. It presents a superior alternative to manual preparation, reducing the workload of embryologists and facilitating the standardization of operational procedures.
摘要：
在人类生育诊所或动物实验室中人工准备试管婴儿的盘子在很大程度上依赖于胚胎学家的经验，这可能导致职业病由于长期和单调的操作。因此,引入自动化技术来取代传统方法对于提高工作效率和减轻胚胎学家的工作负担至关重要。在目前的老鼠研究中，人工和自动化方法均用于制备IVF或胚胎培养皿.进行了单向方差分析，比较了几个因素，包括准备时间,合格费率，菜肴的介质渗透压，受精率,和胚胎发育，以评估自动化制备的效率和潜力。结果表明，自动化系统显著缩短了所需时间，提高了制菜效率和合格率，尤其是胚胎培养皿，没有显著改变中等渗透压。两种制剂在小鼠受精率和胚胎发育方面没有显着差异。因此，自动化培养皿制备可以提高工作效率和合格率，同时保持受精率和随后的胚胎发育，而不影响培养基的渗透压稳定性。它提供了一个优越的替代手工准备，减少胚胎学家的工作量，促进操作程序的标准化。
