PDF(Pubmed)
Abstract：
Exopolysaccharides (EPS) are essential constituents of the extracellular matrix within oral biofilms and are significantly influenced by the local microenvironment. This study aimed to investigate the impact of two distinct antimicrobial agents, DJK-5 and chlorhexidine (CHX), on the EPS volume and pH levels in oral biofilms.
Oral biofilms obtained from two donors were cultured on hydroxyapatite discs for durations of 3 days, 1 week, 2 weeks, 3 weeks, and 4 weeks. Subsequently, these biofilms were subjected to treatment with 10 µg/mL DJK-5 or 2% CHX for 3 min. The impact of these antimicrobial treatments on factors such as the proportion of dead bacterial, in situ pH, and EPS volume within the biofilms was assessed using corresponding fluorescent probes. The examination was carried out utilizing confocal laser scanning microscopy, and the resulting images were analyzed with a focus on the upper and lower layers of the biofilm, respectively.
DJK-5 exhibited a more potent bactericidal effect compared to CHX across the 3-day to 4-week duration of the biofilm (P < 0.05). The biofilms were acidic, with the upper layer being less acidic than the lower layer (P < 0.05). Both antimicrobial agents increased the pH, but DJK-5 had a greater effect than CHX (P < 0.05). The volume of EPS was significantly lower in DJK-5 treated biofilms compared to that of CHX, regardless of age or layer (P < 0.05).
DJK-5 exhibited superior effectiveness in reducing viable bacteria and EPS volume, as well as in raising extracellular pH, as compared to chlorhexidine.
Oral biofilms obtained from two donors were cultured on hydroxyapatite discs for durations of 3 days, 1 week, 2 weeks, 3 weeks, and 4 weeks. Subsequently, these biofilms were subjected to treatment with 10 µg/mL DJK-5 or 2% CHX for 3 min. The impact of these antimicrobial treatments on factors such as the proportion of dead bacterial, in situ pH, and EPS volume within the biofilms was assessed using corresponding fluorescent probes. The examination was carried out utilizing confocal laser scanning microscopy, and the resulting images were analyzed with a focus on the upper and lower layers of the biofilm, respectively.
DJK-5 exhibited a more potent bactericidal effect compared to CHX across the 3-day to 4-week duration of the biofilm (P < 0.05). The biofilms were acidic, with the upper layer being less acidic than the lower layer (P < 0.05). Both antimicrobial agents increased the pH, but DJK-5 had a greater effect than CHX (P < 0.05). The volume of EPS was significantly lower in DJK-5 treated biofilms compared to that of CHX, regardless of age or layer (P < 0.05).
DJK-5 exhibited superior effectiveness in reducing viable bacteria and EPS volume, as well as in raising extracellular pH, as compared to chlorhexidine.
摘要：
背景：胞外多糖（EPS）是口腔生物膜内细胞外基质的必需成分，并且受局部微环境的显着影响。本研究旨在调查两种不同的抗菌药物的影响，DJK-5和氯己定（CHX），对口腔生物膜中EPS体积和pH水平的影响。
方法：将从两个供体获得的口腔生物膜在羟基磷灰石圆盘上培养3天，1周,2周,3周,和4周。随后，这些生物膜用10μg/mLDJK-5或2％CHX处理3分钟。这些抗菌治疗对死亡细菌比例等因素的影响，原位pH值,使用相应的荧光探针评估生物膜内的EPS体积。使用共聚焦激光扫描显微镜进行检查，并将所得图像集中在生物膜的上层和下层进行分析，分别。
结果：DJK-5在生物膜的3天至4周持续时间内表现出比CHX更有效的杀菌作用（P<0.05）。生物膜是酸性的，上层的酸性低于下层(P<0.05)。两种抗菌剂都增加了pH值，但DJK-5的作用大于CHX(P<0.05)。与CHX相比，DJK-5处理的生物膜中EPS的体积显着降低，无论年龄或层（P<0.05）。
结论：DJK-5在减少活菌和EPS体积方面表现出优异的效果，以及提高细胞外pH值，与氯己定相比。
方法：将从两个供体获得的口腔生物膜在羟基磷灰石圆盘上培养3天，1周,2周,3周,和4周。随后，这些生物膜用10μg/mLDJK-5或2％CHX处理3分钟。这些抗菌治疗对死亡细菌比例等因素的影响，原位pH值,使用相应的荧光探针评估生物膜内的EPS体积。使用共聚焦激光扫描显微镜进行检查，并将所得图像集中在生物膜的上层和下层进行分析，分别。
结果：DJK-5在生物膜的3天至4周持续时间内表现出比CHX更有效的杀菌作用（P<0.05）。生物膜是酸性的，上层的酸性低于下层(P<0.05)。两种抗菌剂都增加了pH值，但DJK-5的作用大于CHX(P<0.05)。与CHX相比，DJK-5处理的生物膜中EPS的体积显着降低，无论年龄或层（P<0.05）。
结论：DJK-5在减少活菌和EPS体积方面表现出优异的效果，以及提高细胞外pH值，与氯己定相比。
